SmMYC2b Enhances Tanshinone Accumulation in Salvia miltiorrhiza by Activating Pathway Genes and Promoting Lateral Root Development
Salvia miltiorrhiza Bunge (Lamiaceae) is an economically important medicinal plant as well as an emerging model plant. Our previous studies indicate that Sm MYC2b is a positive transcription factor that can affect the biosynthesis of phenolic acids and tanshinones in S. miltiorrhiza. Moreover, MYC2s...
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Published in | Frontiers in plant science Vol. 11; p. 559438 |
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Main Authors | , , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Frontiers Media S.A
11.09.2020
|
Subjects | |
Online Access | Get full text |
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Summary: | Salvia miltiorrhiza
Bunge (Lamiaceae) is an economically important medicinal plant as well as an emerging model plant. Our previous studies indicate that
Sm
MYC2b is a positive transcription factor that can affect the biosynthesis of phenolic acids and tanshinones in
S. miltiorrhiza.
Moreover, MYC2s are well known to induce the development of lateral roots. As tanshinones are mainly distributed in the periderm, the promotion of lateral root development probably leads to increased accumulation of tanshinones. In this paper, we firstly discovered that
Sm
MYC2b played a dual regulatory role in effectively enhancing the tanshinone accumulation by activating tanshinone biosynthetic pathway and promoting lateral root development. The expression levels of the previously studied pathway genes
SmCPS1
,
SmKSL1
,
SmCYP76AH1
,
SmCYP76AH3
, and
SmCYP76AK1
dramatically increased. In addition,
Sm
MYC2b was proved to exhibit a similar function as other homologs in promoting lateral root development, which increased the tanshinone produced tissue and further enhanced the biosynthesis of tanshinones. RNA-seq assays revealed that
Sm
MYC2b-regulated genes comprised 30.6% (1,901 of 6,210) of JA-responsive genes, confirming that
Sm
MYC2b played a crucial role in transcriptional regulation of JA-regulated genes. Overall, we concluded that
Sm
MYC2b could enhance tanshinone accumulation by activating the tanshinone biosynthetic pathway and promoting lateral root development. Our study provides an effective approach to enhance the production of desired tanshinones and enriches our knowledge of the related regulatory network. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Reviewed by: Guoyin Kai, Zhejiang Chinese Medical University, China; Dongfeng Yang, Zhejiang Sci-Tech University, China Edited by: Goetz Hensel, Heinrich Heine University Düsseldorf, Germany This article was submitted to Plant Biotechnology, a section of the journal Frontiers in Plant Science These authors have contributed equally to this work |
ISSN: | 1664-462X 1664-462X |
DOI: | 10.3389/fpls.2020.559438 |