Supercritical fluid fractionation of thermally oxidized canola oil

• Published in: Journal of the American Oil Chemists' Society Vol. 71; no. 6; pp. 615 - 618
• Main Authors: Hansen, Steven L., Artz, William E.
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer‐Verlag 01.06.1994; Springer
• Subjects: aceite de colza; Biological and medical sciences; calentamiento; chauffage; corps gras; Fat industries; fats; Food industries; fraccionamiento; fractionation; fractionnement; Fundamental and applied biological sciences. Psychology; grasas; Heated oil; heating; high‐performance size‐exclusion chromatography; huile de colza; light‐scattering detector; molecular weight; oxidacion; oxidation; oxydation; peso molecular; poids moleculaire; polymerization; rapeseed oil; supercritical fluid extraction and fractionation; trigliceridos; triglyceride; triglycerides; Separation method; Heat treatment; Extraction process; Carbon dioxide; Gel filtration; Canola oil; Supercritical solvent; Triglyceride
• ISSN: 0003-021X; 1558-9331
• DOI: 10.1007/BF02540588

Supercritical fluid extraction procedures were developed to fractionate thermally oxidized canola oil. Canola oil was heated in a sealed glass ampoule for 72 h at 200°C in a forced‐convection oven. After heating, the percentages of triacylglycerol (TAG) trimer, dimer, monomer and lowermolecular weight (LMW) compounds in the heated oil sample (as determined by high‐performance size‐exclusion chromatography) were 3.0, 8.4, 81.3 and 7.3%, respectively. The LMW compounds included diacylglycerols, monoacylglycerols and free fatty acids. Fractions of TAG monomer of approximately 98% purity were isolated after extraction at 408 atm for 45 min. After a second extraction at 442 atm for 45 min, a TAG dimer fraction of 95% purity was isolated. Recoveries of TAG monomer and dimer were 96 and 87%, respectively.