Sequence of HLA-A6808

• Published in: Tissue antigens Vol. 53; no. 6; pp. 597 - 600
• Main Authors: Cox, S.T., Argüello, J.R., Marsh, S.G.E., Lau, M., Kwan, P.L., Madrigal, J.A., Little, A.-M.
• Format: Journal Article
• Language: English
• Published: Copenhagen Munksgaard International Publishers 01.06.1999
• Subjects: African Continental Ancestry Group - genetics; histocompatibility locus HLA; Histocompatibility Testing - methods; HLA-A Antigens - genetics; HLA-A6808; Humans; Molecular Sequence Data; reference strand conformation analysis; sequence-based typing
• ISSN: 0001-2815; 1399-0039
• DOI: 10.1034/j.1399-0039.1999.530614.x

A novel HLA‐A*68 allele was identified by reference strand conformation analysis of a DNA sample studied for the International Cell Exchange program. Direct sequencing of HLA‐A locus PCR products confirmed the presence of A*6602 and an A*68 allele which differed from A*68011 by two nucleotides at positions 538 and 539. The presence of this sequence motif in this allele which has been named A*6808 was verified by DNA sequencing of full‐length A*6808 clones. No other coding differences between A*68011 and A*6808 were identified. The two nucleotide substitutions found in A*6808 effect an amino acid difference in the encoded protein at residue 156 within the peptide binding site which may evoke allo‐reactive immune response after HLA‐A68 mismatched transplants Note.