Cultivation and Genomics Prove Long-Term Colonization of Donor’s Bifidobacteria in Recurrent Clostridioides difficile Patients Treated With Fecal Microbiota Transplantation
Fecal microbiota transplantation (FMT) is an effective treatment for recurrent Clostridioides difficile infection (rCDI) and it’s also considered for treating other indications. Metagenomic studies have indicated that commensal donor bacteria may colonize FMT recipients, but cultivation has not been...
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Published in | Frontiers in microbiology Vol. 11; p. 1663 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
Frontiers Media S.A
15.07.2020
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Subjects | |
Online Access | Get full text |
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Summary: | Fecal microbiota transplantation (FMT) is an effective treatment for recurrent
Clostridioides difficile
infection (rCDI) and it’s also considered for treating other indications. Metagenomic studies have indicated that commensal donor bacteria may colonize FMT recipients, but cultivation has not been employed to verify strain-level colonization. We combined molecular profiling of
Bifidobacterium
populations with cultivation, molecular typing, and whole genome sequencing (WGS) to isolate and identify strains that were transferred from donors to recipients. Several
Bifidobacterium
strains from two donors were recovered from 13 recipients during the 1-year follow-up period after FMT. The strain identities were confirmed by WGS and comparative genomics. Our results show that specific donor-derived bifidobacteria can colonize rCDI patients for at least 1 year, and thus FMT may have long-term consequences for the recipient‘s microbiota and health. Conceptually, we demonstrate that FMT trials combined with microbial profiling can be used as a platform for discovering and isolating commensal strains with proven colonization capacity for potential therapeutic use. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 These authors have contributed equally to this work Reviewed by: Stefano Raimondi, University of Modena and Reggio Emilia, Italy; Rodrigo Bacigalupe, VIB KU Leuven Center for Microbiology, Belgium; Roderick Ian Mackie, University of Illinois at Urbana-Champaign, United States This article was submitted to Microbial Symbioses, a section of the journal Frontiers in Microbiology Edited by: Tom Van De Wiele, Ghent University, Belgium |
ISSN: | 1664-302X 1664-302X |
DOI: | 10.3389/fmicb.2020.01663 |