The human T cell transcription factor-1 gene. Structure, localization, and promoter characterization
We have recently isolated cDNA clones representing four alternative splice forms of a T cell-specific transcription factor, TCF-1. Here we report the characterization of the human gene encoding this factor. The TCF-1 gene is contained in 10 exons including an untranslated first exon. The DNA-binding...
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Published in | The Journal of biological chemistry Vol. 267; no. 12; pp. 8530 - 8536 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
Bethesda, MD
American Society for Biochemistry and Molecular Biology
25.04.1992
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Subjects | |
Online Access | Get full text |
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Summary: | We have recently isolated cDNA clones representing four alternative splice forms of a T cell-specific transcription factor,
TCF-1. Here we report the characterization of the human gene encoding this factor. The TCF-1 gene is contained in 10 exons
including an untranslated first exon. The DNA-binding high mobility group (HMG) box of TCF-1 is encoded by the closely spaced
exons VI and VII. Differential splicing involves an alternative exon (IX) and three splice acceptor sites in exon X. Based
on comparison of sequence and on the placement of an alternative exon, TCF-1 appears closely related to the recently characterized
HMG box transcription factor TCF-1 alpha/LEF. In particular, the HMG boxes encoded by the two TCF genes are virtually identical.
The TCF-1 gene resides on chromosome 5 band q31.1. The TCF-1 promoter coincides with a CpG island. As determined by chloramphenicol
acetyltransferase analysis, the promoter is preferentially active in T cells. The promoter does not contain TCF-1/TCF-1 alpha
binding sites and is therefore not autoregulated. This observation implies the existence of yet uncharacterized T cell transcription
factors that are active during early T cell differentiation. |
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Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 |
ISSN: | 0021-9258 1083-351X |
DOI: | 10.1016/S0021-9258(18)42476-3 |