Target Extension‐Activated DNA Walker on Nanoparticles for Digital Counting‐Based Analysis of MicroRNA

Main observation and conclusion MicroRNAs (miRNAs), especially exosomal miRNAs, are promising noninvasive biomarkers in early‐stage cancer diagnosis and disease treatment monitoring. However, their precise and sensitive quantification remains challenging due to their small size and low abundance. He...

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Bibliographic Details
Published inChinese journal of chemistry Vol. 39; no. 6; pp. 1471 - 1476
Main Authors Feng, Qinya, Zhai, Yuqing, Ren, Wei, Liu, Chenghui
Format Journal Article
LanguageEnglish
Published Weinheim WILEY‐VCH Verlag GmbH & Co. KGaA 01.06.2021
Wiley Subscription Services, Inc
Subjects
Biomarkers
Deoxyribonucleic acid
Digital counting
DNA
DNA cleavage
Fluorescence
Medical treatment
MicroRNAs
miRNA
Nanoparticles
RNA recognition
Total internal reflection fluorescent microscope
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Summary:Main observation and conclusion MicroRNAs (miRNAs), especially exosomal miRNAs, are promising noninvasive biomarkers in early‐stage cancer diagnosis and disease treatment monitoring. However, their precise and sensitive quantification remains challenging due to their small size and low abundance. Herein, we have developed a nanoparticle‐confined DNA walker strategy for the specific detection of miRNA. In the existence of the target miRNA, the on‐particle DNA walking reaction will be initiated, providing a fluorescence‐positive nanoparticle. Otherwise, the nanoparticle would be fluorescence‐negative. Utilizing the total internal reflection fluorescent microscope (TIRFM) to digitally count the fluorescence‐positive nanoparticles, the proposed method possesses a detection limit of 0.2 pmol/L miRNA and can accurately distinguish the single‐base mismatched target. This design combines the merits of the DNA walker for signal amplification and the TIRFM for highly sensitive detection, paving a new way for the digital counting‐based analysis of exosomal miRNAs. A new DNA walking mechanism is developed for the digital counting‐based analysis of miRNA.
Bibliography:†
In Situ
Target Biomolecule Analysis in Confined Nanospace.
Dedicated to the Special Issue of
ISSN:1001-604X
1614-7065
DOI:10.1002/cjoc.202000692