Two Japanese quail [Coturnix japonica] lines selected for high and low antibody response were homozygous at major histocompatibility complex

• Published in: The Journal of Poultry Science Vol. 42; no. 3; pp. 272 - 281
• Main Authors: Koyama, T.(National Inst. of Animal Health, Tsukuba, Ibaraki (Japan)), Miura, K, Inooka, S, Takahashi, S
• Format: Journal Article
• Language: English
• Published: Ibaraki Japan Poultry Science Association 2005; Japan Science and Technology Agency
• Subjects: ANTIBODIES; ANTICORPS; ANTICUERPOS; CAILLE; CODORNIZ; HIBRIDACION; HOMOCIGOTOS; HOMOZYGOTE; HOMOZYGOTES; HYBRIDATION; HYBRIDIZATION; INBRED LINES; Japanese quail; LIGNEE CONSANGUINE; LINEAS CONSANGUINEAS; MAJOR HISTOCOMPATIBILITY COMPLEX; MHC homozygous line; POLIMORFISMO; POLYMORPHISM; POLYMORPHISME; QUAILS; SISTEMA MHC; Southern hybridization; SYSTEME MHC
• ISSN: 1346-7395; 1349-0486
• DOI: 10.2141/jpsa.42.272

In the present study, Southern hybridization analysis was used to investigate whether segregated major histocompatibility complex (MHC) genes were selected in two partly inbred Japanese quail (Coturnix japonica) lines, the H and L lines, which were selected for high (H) and low (L) antibody response against inactivated Newcastle disease virus (NDV) vaccine as a result of breeding over 45 generations by reciprocal mating. The results showed that the each restriction fragments length polymorphisms (PFLP) pattern of MHC class I, 11 and IV of all the individuals within the H and L lines were identical. The RFLP patterns of MHC class II in F1 of spring resulting from the H and L lines were inherited equally. This suggested that divergent selection for antibody response to inactivated NDV in Japanese quails might have induced two difflerent MHC-homozygous lines.