The disappearance rate and risk assessment of thiacloprid residues in Asian pear using liquid chromatography confirmed with tandem mass spectrometry

• Published in: Biomedical chromatography Vol. 31; no. 5; pp. np - n/a
• Main Authors: Kabir, Md. Humayun, Abd El‐Aty, A.M., Rahman, Md. Musfiqur, Kim, Sung‐Woo, Choi, Jeong‐Heui, Lee, Young‐Jun, Truong, Lieu T.B., Lee, Kang‐Bong, Kim, Mi‐Ra, Shin, Ho‐Chul, Shim, Jae‐Han
• Format: Journal Article
• Language: English
• Published: England 01.05.2017
• Subjects: Asian pear; Calibration; Chromatography, Liquid - methods; dissipation kinetic; Food Analysis - methods; Food Contamination - analysis; Kinetics; LC‐UVD; Limit of Detection; Neonicotinoids; Pesticide Residues - analysis; PHRL; Pyridines - analysis; Pyrus - chemistry; Risk Assessment; Sensitivity and Specificity; Tandem Mass Spectrometry - methods; thiacloprid; Thiazines - analysis; thiacloprid; risk assessment; LC-UVD; Asian pear; dissipation kinetic; PHRL
• ISSN: 0269-3879; 1099-0801
• DOI: 10.1002/bmc.3861

This study was undertaken to quantify the residue levels and propose the dissipation kinetics of thiacloprid formulated as suspension concentrate in field‐incurred Asian pears grown under two different open‐field conditions. Samples were extracted with 20% distilled water in acetonitrile; partitioned with brine water and dichloromethane; and purified with a Florisil solid phase extraction cartridge. The analyte was identified with an LC ultraviolet detector, and field‐incurred samples were confirmed using LC–MS/MS. The calibration curve was linear over the range 0.05–5.0 mg/L with a satisfactory coefficient of determination (R2 = 0.9994). The limits of detection and limits of quantification (LOQ) were 0.003 and 0.01 mg/kg, respectively. The recovery rate fortified to blank samples at LOQ, 10× LOQ, and the maximum residue limit (MRL) were between 73.7 and 86.2% with relative standard deviation ≤9.0%. The residual concentrations at both sites were considerably lower than the MRL (0.7 mg/kg) set by the Korean Ministry of Food Drug Safety, with biological half‐lives of 5.0 and 7.4 days, for sites 1 and 2, respectively. From the pre‐harvest residue limit curve, it was predicted that if the residues were <1.13 or 1.40 mg/kg 10 days before harvest, the residue level would be lower than the MRL during harvest. Risk assessment on day 0 showed an acceptable daily intake (%) of 13.0% and 11.0% for sites 1 and site 2, respectively, which indicates that the residual amounts are not hazardous to the Korean population.