Anti-CD74 IgA antibodies show diagnostic potential for axial spondyloarthritis but are not associated with microscopic gut inflammation

• Published in: Rheumatology (Oxford, England) Vol. 62; no. 2; pp. 984 - 990
• Main Authors: De Craemer, Ann-Sophie, Witte, Torsten, Lobaton Ortega, Triana, Hoorens, Anne, De Vos, Martine, Cuvelier, Claude, Vastert, Sebastiaan J, Baraliakos, Xenofon, Van den Bosch, Filip, Elewaut, Dirk
• Format: Journal Article
• Language: English
• Published: England 01.02.2023
• Subjects: Axial Spondyloarthritis; Fibromyalgia; Humans; Immunoglobulin A; Inflammation; Magnetic Resonance Imaging; Spondylarthritis - diagnosis; Spondylitis, Ankylosing - diagnosis; diagnosis; anti-CD74 antibodies; gut inflammation; biomarker; axial spondyloarthritis
• ISSN: 1462-0324; 1462-0332
• DOI: 10.1093/rheumatology/keac384

Gut inflammation commonly occurs in axial SpA (axSpA), and is linked to disease activity and outcome. Given the role of IgA in mucosal immunity, we explored the association between anti-CD74 IgA antibodies, gut inflammation and axSpA. Anti-CD74 IgA was measured by ELISA in serum samples of axSpA patients, fulfilling the 2009 Assessment of SpondyloArthritis international Society classification criteria. A group of fibromyalgia (FM) and RA patients served as non-inflammatory and inflammatory controls. Newly diagnosed axSpA patients underwent ileocolonoscopy; mucosal biopsies were histopathologically assessed as normal, acute or chronically inflamed. Optimal anti-CD74 IgA cut-off values were determined with a receiver operating characteristics curve. axSpA patients (n = 281) showed higher anti-CD74 IgA levels [mean (s.d.) 18.8 (12.4) U/ml] compared with 100 FM patients [10.9 (5.0) U/ml, P < 0.001] and 34 RA patients [13.7 (9.6) U/ml, P = 0.02]. The area under the receiver operating characteristics curve for diagnosis (axSpA vs FM) was 0.70, providing a sensitivity of 60% and specificity of 87% (cut-off 15 U/ml). Antibody concentrations were not significantly different between axSpA patients with (n = 40) and without (n = 69) gut inflammation (P = 0.83), yielding an area under the receiver operating characteristics curve of 0.51. Anti-CD74 IgA levels were not associated with degree of bone marrow oedema on MRI of the sacroiliac joints, CRP or any other disease-specific feature such as the use of NSAIDs or biological treatment. Serum anti-CD74 IgA is a potentially useful diagnostic biomarker for axSpA. However, antibody levels do not correlate with any phenotypical feature, including microscopic gut inflammation, suggesting this to be a disease-specific rather than an inflammatory marker.