Higher ANGPTL3, apoC-III, and apoB48 dyslipidemia, and lower lipoprotein lipase concentrations are associated with dysfunctional visceral fat in adolescents with obesity

• Published in: Clinica chimica acta Vol. 508; pp. 61 - 68
• Main Authors: Rodríguez-Mortera, Reyna, Caccavello, Russell, Garay-Sevilla, Ma. Eugenia, Gugliucci, Alejandro
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.09.2020
• Subjects: Adolescent; Angiopoietin-Like Protein 3; Angiopoietin-like Proteins; apoB48; apoC-III; Apolipoprotein B-48; Apolipoprotein C-III; Atherosclerosis; Chylomicron remnants; Cross-Sectional Studies; Dyslipidemias; Humans; Insulin-resistance; Intra-Abdominal Fat; LDL subclasses; Lipoprotein Lipase; Metabolic syndrome; Obesity; Triglycerides; T2DM; NAFLD; apoC-III; HL; apoB48; Chylomicron remnants; LPL; Lipoprotein lipase; Metabolic syndrome; sd-LDL; WC; BP; Insulin-resistance; TRL; DBP; Atherosclerosis; AGE; SBP; Obesity; LDL subclasses; AI; Angiopoietin-like proteins; IR; CR; ANGPTL 3, 4, 8; MetS; sRAGE; HC; CVR; VAI
• ISSN: 0009-8981; 1873-3492
• DOI: 10.1016/j.cca.2020.05.014

•Adolescents with obesity show higher ANGPTL3 compounded with increased apoC-III.•They have increased levels of apoB48 (chylomicrons and their remnants)•They have decreased LPL mass.•The resulting LPL inhibition impairs VLDL and chylomicron catabolism leading to atherogenic remnants. We hypothesized that adolescents with obesity have higher remnant B48 concentrations associated with lipoprotein lipase dysregulation. Cross-sectional study of 32 adolescents with obesity and 27 control subjects. As compared to lean controls, obese participants showed 35% higher concentrations of apoB48: 3.60 (2.93–4.30) vs 2.65 (1.64–3.68) ng/ml; 28% of apoC-III: (72.7 (58.6–89.7) vs 56.9 (44.8–79.8 ug/ml and 17% ANGPTL 3: (72.2 ± 20.2 vs 61.2 ± 19.2 ng/ml). This was accompanied by a 33% reduction in LPL: 13.1 ± 5.1 vs 18.9 ± 4.7 ng/ml. Obese participants had 25% lower adiponectin 2.9 (1.9–3.8) vs 4.4 (3.2.-5.2) μg/ml; 260% higher leptin 25.7 (11.2–44.8) vs 9.3 (2.8–20.7) ng/ml c and 83% higher Il-6: 2.2 (1.3–5.4) vs 1.2 (0.8–1.4) pg/ml. ApoC-III and ANGPTL3 correlated positively with VAI; ANGPTL3 negatively with HDL-C; LDL size and VLDL-C. ApoB48 correlated negatively with LDL-C. Adolescents with obesity show higher ANGPTL3 compounded with increased apoC-III associated with increased CR and lower LPL mass. This is associated with inflammation and visceral fat. The significance of these findings resides in that they shed light on a mechanism for TRL dyslipidemia in adolescents: increased LPL inhibition impairs VLDL and chylomicron catabolism leading to atherogenic remnants.