Iron uptake by melanoma cells from the soluble form of the transferrin homologue, melanotransferrin

Melanotransferrin (MTf) is a membrane-bound transferrin (Tf) homologue that can also exist in a soluble form (sMTf). Considering the high homology of MTf to Tf, it is possible to suggest that sMTf could bind to the high affinity transferrin receptor 1 (TfR1) or lower affinity TfR2. We have used sMTf...

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Bibliographic Details
Published inRedox report : communications in free radical research Vol. 7; no. 5; pp. 279 - 282
Main Authors Food, Michael R., Richardson, Des R.
Format Journal Article
LanguageEnglish
Published England Taylor & Francis 01.01.2002
Subjects
Antigens, Neoplasm
Binding Sites
Binding, Competitive
Humans
Iron - metabolism
Iron - pharmacokinetics
Melanoma - metabolism
Melanoma-Specific Antigens
Neoplasm Proteins - metabolism
Temperature
Time Factors
Transferrin - metabolism
Tumor Cells, Cultured
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Summary:Melanotransferrin (MTf) is a membrane-bound transferrin (Tf) homologue that can also exist in a soluble form (sMTf). Considering the high homology of MTf to Tf, it is possible to suggest that sMTf could bind to the high affinity transferrin receptor 1 (TfR1) or lower affinity TfR2. We have used sMTf labelled with 59 Fe to examine its ability to donate Fe to cells. Our experiments demonstrate that sMTf is far less effective than Tf at donating Fe to cells and this does not occur via specific receptors. Indeed, the uptake of sMTf by cells occurred via a non-specific process (e.g. adsorptive pinocytosis).
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ISSN:1351-0002
1743-2928
DOI:10.1179/135100002125000794