Total flavonoids of sea buckthorn (Hippophae rhamnoides L.) improve MC903-induced atopic dermatitis-like lesions

• Published in: Journal of ethnopharmacology Vol. 292; p. 115195
• Main Authors: Gu, Yajing, Wang, Xinxin, Liu, Fang, Zhang, Jin, Zhang, Xiufang, Liu, Jiping, Li, Sijia, Wang, Decheng, Guan, Hongquan, Hou, Diandong
• Format: Journal Article
• Language: English
• Published: Ireland Elsevier B.V 28.06.2022
• Subjects: Animals; Anti-allergic; Anti-inflammatory; Anti-Inflammatory Agents - adverse effects; Atopic dermatitis; Cytokines - genetics; Cytokines - metabolism; Dermatitis, Atopic - chemically induced; Dermatitis, Atopic - drug therapy; Dinitrochlorobenzene; Flavonoids - pharmacology; Flavonoids - therapeutic use; Hippophae; Interleukin-4 - metabolism; Mice; Mice, Inbred C57BL; NF-kappa B - metabolism; Total flavonoids of Hippophae rhamnoides L; Tumor Necrosis Factor-alpha - pharmacology; Anti-allergic; Total flavonoids of Hippophae rhamnoides L; Atopic dermatitis; Anti-inflammatory
• ISSN: 0378-8741; 1872-7573
• DOI: 10.1016/j.jep.2022.115195

Sea buckthorn (Hippophae rhamnoides L.) is popularly used as a herbal medicine and food additive in the world. Total flavonoids of Hippophae rhamnoides (TFH) are reported to have anti-inflammatory and immunomodulatory activities. The effects of TFH on atopic dermatitis (AD)-like lesions induced by MC903 in mice was elucidated in the study. To induce AD-like lesions, MC903 was adopted to apply repeatedly on the left ear in C57BL/6 mice. After induction of AD-like lesions, 0.5% and 1% TFH cream was applied topically on ears of mice once a day for 8 days. The degree of skin lesions was evaluated by macroscopical and histological methods. Expressions of filaggrin (FLG) was evaluated by Western blotting. Real-time polymerase chain reaction (qPCR) was adopted to detect the mRNA expression of thymic stromal lymphopoietin (TSLP), interferon (IFN)-γ, interleukin (IL-4), tumor necrosis factor (TNF)-α in skin lesions. In vitro, Cytokine Antibody Arrays were performed to measure production of cytokines in IFN-γ/TNF-α-treated HaCaT cells, Western blotting was employed to detect the expressions of p–NF–κB, p-ERK and p-P38. Topical application of TFH significantly improved the severity of dermatitis by inhibiting the infiltration of mast cell, increasing expression of FLG, decreasing the expressions of TNF-α, IL-4, IFN-γ and TSLP in skin lesions. TFH decreased the levels of IL-1α, IL-1β, IL-6, monocyte chemoattractant protein (MCP)-1, MCP-3, macrophage-derived chemokine (MDC), platelet-derived growth factor (PDGF)-BB, thymus and activation regulated chemokine (TARC) in the supernatants of the HaCaT cells treated by IFN-γ/TNF-α. Furthermore, expressions of p–NF–κB, p-ERK and p-P38 were also decreased by TFH administration with dose dependent manner in HaCaT cells treated by IFN-γ/TNF-α. Topical application of TFH improved AD-like lesions in mice induced by MC903. Which exerted the effects of anti-inflammation and repairing skin barrier by regulating Th1/Th2 balance. This finding indicates that TFH is a novel potential agent for the external treatment of AD. [Display omitted]