Volatile components and antibacterial effects of pine needle (Pinus densiflora S. and Z.) extracts

• Published in: Food microbiology Vol. 22; no. 1; pp. 37 - 45
• Main Authors: Kim, Yong-Suk, Shin, Dong-Hwa
• Format: Journal Article
• Language: English
• Published: London Elsevier Ltd 01.01.2005; Elsevier
• Subjects: Antibacterial activity; Bacillus cereus; Biological and medical sciences; Escherichia coli; Food industries; Food microbiology; Foodborne bacteria; Fundamental and applied biological sciences. Psychology; Pinus densiflora; Salmonella typhimurium; SDE; Staphylococcus aureus; Volatile components; Antibacterial activity; Volatile components; Pinus densiflora; SDE; Foodborne bacteria; Volatile compound; Gymnospermae; Coniferales; Spermatophyta; Chemical composition; Extract; Antibacterial agent
• ISSN: 0740-0020; 1095-9998
• DOI: 10.1016/j.fm.2004.05.002

The antibacterial effects of the volatile components extracted from Pinus densiflora S. and Z., by simultaneous steam distillation and solvent extraction (SDE), were examined on six foodborne bacteria using Bioscreen C (a computer-controlled shake–incubator–reader). The SDE extracts of P. densiflora obtained after 1.5 or 2.0h at pH 3.6 exhibited a strong growth inhibitory effect on Escherichia coli O157:H7 and their overall antibacterial activities against the various bacteria tested tended to increase with increased extraction time and with a lower extraction pH. The major volatile components of the SDE extracts obtained at pH 3.6 and 1.5h, as determined by gas chromatography, were α-ocimene (29.3%), sabinene (10.9%), β-myrcene (9.6%), β-caryophyllene (8.0%), β-cadinene (7.3%), α-terpinolene (4.9%), 2-hexanal (4.5%), and β-pinene (4.3%). The addition of 8% or 10% (v/v) of the SDE extracts to culture broth completely inhibited the growths of Bacillus cereus, Salmonella Typhimurium, and Staphylococcus aureus. The intracellular adenosine triphosphate (ATP) concentration of S. Typhimurium treated with P. densiflora extract reduced to 0.165μm from 0.595μm, whereas the ATP concentration in culture supernatants was increased to 0.469μm form 0.065μm.