Salivary MMPs, TIMPs, and MPO levels in periodontal disease patients and controls

Matrix metalloproteinases (MMPs) are a family of zinc-dependent endopeptidases with an important role in physiological and pathological remodeling. Their activity is regulated by tissue inhibitors of matrix metalloproteinases (TIMPs). Excess MMPs and myeloperoxidase (MPO) activity have been associat...

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Published inClinica chimica acta Vol. 421; pp. 140 - 146
Main Authors Meschiari, Cesar A., Marcaccini, Andrea M., Santos Moura, Bruna C., Zuardi, Leonardo R., Tanus-Santos, Jose E., Gerlach, Raquel F.
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 05.06.2013
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Summary:Matrix metalloproteinases (MMPs) are a family of zinc-dependent endopeptidases with an important role in physiological and pathological remodeling. Their activity is regulated by tissue inhibitors of matrix metalloproteinases (TIMPs). Excess MMPs and myeloperoxidase (MPO) activity have been associated with loss of tooth supporting tissues in periodontal disease (PD). We investigate the changes in salivary MMP-8, MMP-9, TIMP-1, TIMP-2, and MPO concentrations during PD treatment and compare results with plasma levels. MMP-8, MMP-9, TIMP-1 and TIMP-2 were analyzed by ELISA. Gelatinolytic activity of MMP-9 forms was determined by zymography, and the MPO activity was determined by colorimetric assay. Subjects were divided into 2 groups: PD and control, which were further divided into 2 subgroups each, namely PD before (PB) and after 3months (PA) of non-surgical periodontal therapy, and healthy volunteers at baseline (CB) and 3months after baseline (CA). Subgroup PA presented lower gelatinolytic activity and MMP-8 and TIMP-2 concentrations in the saliva compared with PB (p<0.05). The MPO activity was higher in PB compared with CB (p<0.05). There were significant correlations between the gelatinolytic activity of the saliva and MMP-8 and MMP-9 plasma levels. There was a significant correlation between plasma and saliva TIMP-2 levels. These results suggest attenuation of some inflammatory markers in the saliva and plasma after PD treatment. Moreover, correlations between salivary and plasma levels exist for some of these markers. •Inflammatory markers decreased in the saliva after periodontal disease treatment.•We observed positive correlations between salivary inflammatory marker levels.•We observed correlations between salivary and plasma inflammatory marker levels.•MPO activity in the plasma is not altered by periodontal disease or the treatment.
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ISSN:0009-8981
1873-3492
DOI:10.1016/j.cca.2013.03.008