Rapid detection of chlorpyrifos pesticide residue in tea using surface-enhanced Raman spectroscopy combined with chemometrics

• Published in: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy Vol. 250; p. 119366
• Main Authors: Zhu, Xiaoyu, Li, Wenjin, Wu, Ruimei, Liu, Peng, Hu, Xiao, Xu, Lulu, Xiong, Zhengwu, Wen, Yangping, Ai, Shirong
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 05.04.2021
• Subjects: Chlorpyrifos; Competitive adaptive reweighted sampling; Gold; Metal Nanoparticles; Pesticide Residues - analysis; Rapid pretreatment; Spectrum Analysis, Raman; Support vector machine; Surface-enhanced Raman spectroscopy; Tea; Chlorpyrifos; Tea; Support vector machine; Rapid pretreatment; Competitive adaptive reweighted sampling; Surface-enhanced Raman spectroscopy
• ISSN: 1386-1425; 1873-3557
• DOI: 10.1016/j.saa.2020.119366

[Display omitted] •AuNPs were synthesized by reducing gold using sodium citrate.•QuEChERS method as an effective pretreatment technology for tea sample.•Chemometrics methods have been employed to develop quantitative analysis model. Surface enhanced Raman spectroscopy based on rapid pretreatment combined with Chemometrics was used to determine chlorpyrifos residue in tea. Au nanoparticles were used to as enhance substrate. Different dosages of PSA and NBC were investigated to eliminate the tea substrate influence. Competitive adaptive reweighted sampling (CARS) was used to optimize the characteristic peaks, and compared to full spectra variables and the experiment selected variables. The results showed that PSA of 80 mg and NBC of 20 mg was an excellent approach for rapid detecting. CARS - PLS had better accuracy and stability using only 1.7% of full spectra variables. SVM model achieved better performance with R2p = 0.981, RMSEP = 1.42 and RPD = 6.78. Recoveries for five unknown concentration samples were 98.47 ~ 105.18% with RSD − 1.53% ~ 5.18%. T-test results showed that t value was 0.720, less than t0.05,4 = 2.776, demonstrating that no clear difference between the real value and predicted value. The detection time of a single sample is completed within 15 min. This study demonstrated that SERS coupled with Chemometrics and QuEChERS may be employed to rapidly examine the chlorpyrifos residue in tea towards its quality and safety monitoring.