Genetic relationships and diversity within cultivated accessions of Salvia officinalis L. in Turkey

• Published in: Plant biotechnology reports Vol. 15; no. 5; pp. 663 - 672
• Main Authors: Tursun, Ayse Ozlem, Sipahioglu, Hikmet Murat, Telci, Isa
• Format: Journal Article
• Language: English
• Published: Singapore Springer Singapore 01.10.2021; Springer Nature B.V; 한국식물생명공학회
• Subjects: Agriculture; Amplification; Biomedical and Life Sciences; Biotechnology; Cell Biology; Divergence; Flora; Genetic diversity; Genetic relationship; Genotypes; Life Sciences; Nucleotide sequence; Original Article; Phylogeny; Plant Biochemistry; Plant Sciences; Ribosomal DNA; Salvia officinalis; 농학; Turkey; ITS; spp; Genetic marker; Variation; PCR
• ISSN: 1863-5466; 1863-5474
• DOI: 10.1007/s11816-021-00712-2

Sage ( Salvia officinalis L.) is not found in Turkey's natural flora; however, its cultivation has been increasing in recent years. The species is widely cultivated in many provinces of the Aegean and the Mediterranean regions. There has been no information regarding genetic characteristics and the intra-individual phylogeny of cultivated sage. The levels of genetic relatedness of 19 Salvia samples from Izmir were determined by molecular tools. The sequences from the internal transcriber spacer (ITS) region of 18S–28S nuclear ribosomal DNA (nrDNA) locus were amplified by PCR and sequenced. Approximately, a 710 bp single amplified product was obtained in all genotypes. Of the nineteen Salvia specimens, six were identified as Salvia x sylvestris L. and the remaining 13 specimens were S. officinalis. The sequences of both species shared no similar secondary structures. The ITS region of all specimens contains several indels and substitutions. In the phylogenetic dendrogram, three major clusters (I, II, and III) and two independent branches (IV and V) were observed. Ninety-seven percent of local Salvia specimens were clustered in two branches (I and II), indicating a high diversity of genetic relatedness. Sequence divergence was higher among the specimens of S. officinalis than the specimens of Salvia x sylvestris .