Influences of Mo on Nitrate Reductase, Glutamine Synthetase and Nitrogen Accumulation and Utilization in Mo-Efficient and Mo-Inefficient Winter Wheat Cultivars

• Published in: Agricultural sciences in China Vol. 9; no. 3; pp. 355 - 361
• Main Authors: YU, Min, HU, Cheng-xiao, SUN, Xue-cheng, WANG, Yun-hua
• Format: Journal Article
• Language: English
• Published: Research Center of Trace Elements,Huazhong Agricultural University,Wuhan 430070,P.R.China 01.03.2010; Department of Horticulture,Foshan University,Foshan 528000,P.R.China%Research Center of Trace Elements,Huazhong Agricultural University,Wuhan 430070,P.R.China
• Subjects: Mo; nitrate reductase; N accumulation; winter wheat; N use efficiency; glutamine synthetase
• ISSN: 1671-2927
• DOI: 10.1016/S1671-2927(09)60104-8

S1; The objective is to study whether the accumulation and utilization of plant N are controlled by Mo status in winter wheat cultivars. Mo-efficient cultivar 97003 (eff) and Mo-inefficient cultivar 97014 (ineff) were grown in severely Mo-deficient acidic soil (Tamm-reagent-extractable Mo 0.112 mg kg-1) with (+Mo) and without (-Mo) the application of 0.13 mg kg-1 Mo.The accumulation and use efficiency of plant total N were significantly higher in +Mo than that in -Mo and in eff than that in ineff under Mo deficiency. N use efficiency was remarkably higher in maturity but it was forwarded to jointing stage after Mo supply,thus indicating that Mo supply promoted the N use efficiency besides N uptake and eft was efficient in N uptake and utilization. The overall activity of nitrate reductase (NR,EC 1.6.6.1) was significantly higher in +Mo than in -Mo and ratio of +Mo/-Mo was even to 14.8 at tillering stage for ineff. Activity of glutamine synthetase (GS,EC 6.3.1.2) was significantly lower in +Mo than in -Mo. Concentration of nitrate and glutamate were also significantly lower in +Mo than in -Mo,thus provided evidences for enhancing N use efficiency by Mo supply. Activities of NR and GS were significantly higher and concentrations of nitrate and glutamate were significantly lower in elf than ineff under Mo deficiency,thus indicated eft was more efficient in N reduction and utilization. It is therefore concluded that Mo could promote N accumulation and utilization in winter wheat which was directly related to NR and feedback regulated by GS.Higher Mo status also results in higher accumulation and utilization of plant N in eff.