Molecular cloning, expression, and mapping of a novel human cDNA, GRP17, highly homologous to human gadd45 and murine MyD118

• Published in: Journal of human genetics Vol. 44; no. 5; pp. 300 - 303
• Main Authors: Suzuki, M, Watanabe, T K, Fujiwara, T, Nakamura Yp6, Takahashi, E, Tanigami, A
• Format: Journal Article
• Language: English
• Published: England 01.01.1999
• Subjects: Amino Acid Sequence; Animals; Antigens, Differentiation - biosynthesis; Antigens, Differentiation - chemistry; Antigens, Differentiation - genetics; Base Sequence; Carrier Proteins; Chromosome Mapping; Chromosomes, Human, Pair 9; Cloning, Molecular; DNA Damage; DNA, Complementary - genetics; Female; GADD45 Proteins; Humans; In Situ Hybridization, Fluorescence; Intracellular Signaling Peptides and Proteins; Male; Mice; Molecular Sequence Data; Organ Specificity; Pregnancy; Proteins - chemistry; Proteins - genetics; Recombinant Proteins - biosynthesis; Recombinant Proteins - chemistry; Sequence Alignment; Sequence Homology, Amino Acid; Transcription, Genetic
• ISSN: 1434-5161; 1435-232X
• DOI: 10.1007/s100380050164

From a human placenta cDNA library, we isolated a novel gene whose predicted product is highly homologous in amino acid sequence to human gadd45 and murine MyD118 proteins (about 55% and 52% identity, respectively). The cDNA clone, designated GRP17 (gadd-related protein, 17 kDa), contained a 477-bp open reading frame encoding 159 amino acids. Northern blot analysis revealed strong expression of a 1.35-kb transcript in heart, placenta, liver, skeletal muscle, prostate, testis, and ovary. A 1.7-kb additional transcript was detected in liver. We assigned the GRP17 gene to chromosome 9q22.1-q22.2 by fluorescence in situ hybridization (FISH).