Antidiabetic properties of lyophilized extract of acorn (Quercus brantii Lindl.) on experimentally STZ-induced diabetic rats

• Published in: Journal of ethnopharmacology Vol. 176; pp. 243 - 251
• Main Authors: Dogan, Abdulahad, Celik, Ismail, Kaya, Mehmet Salih
• Format: Journal Article
• Language: English
• Published: Ireland Elsevier Ireland Ltd 24.12.2015
• Subjects: Acorn; Alanine Transaminase - blood; Alkaline Phosphatase - blood; Animals; Antioxidant defense system; Aspartate Aminotransferases - blood; Blood Glucose - analysis; Brain - drug effects; Brain - metabolism; Diabetes mellitus; Diabetes Mellitus, Experimental - blood; Diabetes Mellitus, Experimental - drug therapy; Erythrocytes - drug effects; Erythrocytes - metabolism; Freeze Drying; Glutathione Transferase - metabolism; Glycoside Hydrolases - metabolism; Hyperglycemia - blood; Hyperglycemia - drug therapy; Hypoglycemic Agents - chemistry; Hypoglycemic Agents - therapeutic use; Hypoglycemic Agents - toxicity; Intestine, Small - drug effects; Intestine, Small - metabolism; Kidney - drug effects; Kidney - metabolism; Lipid Metabolism - drug effects; Liver - drug effects; Liver - metabolism; Male; Malondialdehyde; Malondialdehyde - metabolism; Nuts; Oxidative stress; Phytotherapy; Plant Extracts - chemistry; Plant Extracts - therapeutic use; Plant Extracts - toxicity; Quercus; Rats; Rats, Wistar; Serum biomarkers; 5,5′-dithiobis-(2-nitrobenzoic acid) (DTNB) (PubChem CID: 6254); Oxidative stress; Butylated hydroxytoluene (BHT) (PubChem CID: 31404); Streptozotocin (STZ) (PubChem CID: 29327); LP; ALP; DM+AC; ALT; DM; MDA; Ethylenediaminetetraacetic acid (EDTA) (PubChem CID: 6049); 1-chloro-2,4-dinitrobenzene (CDNB) (PubChem CID: 6); LDH; QB; Trichloroacetic acid (TCA) (PubChem CID: 6421); STZ; GSH; HbA1c; AST; GSH-Px; β-Nicotinamide adenine dinucleotide phosphate (NADPH) (PubChem CID: 5884); LDL-c; Diabetes mellitus; Rats; Oxidized glutathione (GSSG) (PubChem CID: 65359); GST; HRDB; LDSE; SOD; Acorn; Serum biomarkers; GR; ADSC; Malondialdehyde; Antioxidant defense system; TC; HDL-c; Thiobarbituric acid (TBA) (PubChem CID: 2723628); Reduced glutathione (GSH) (PubChem CID: 124886); DM+QB; TG; CAT; CRE; DB
• ISSN: 0378-8741; 1872-7573
• DOI: 10.1016/j.jep.2015.10.034

Acorn obtained from the Quercus brantii Lindl. (QB) tree has been used in Turkish folk medicine. Some studies have reported as an antioxidant, antimicrobial, anti-inflammatory, gastroprotective, antitumoural of QB properties previously. however, its effect on the management of type diabetic 2 and oxidative stress complications is still unexplored. The aims of our study were the evaluation of the protective effect and antioxidant role of acorn lyophilized seed plant extract against STZ-induced diabetic complications as oxidative stress, hepatotoxicity and nephropathy, lipidemia and serum biomarkers of diabetes (SBD). In-vivo studies were performed on STZ-induced diabetic rats Experiment was designed as I [Normal Control (NC)], II [Diabetes mellitus (DM)], III [DM+Acarbose (20mg/kg b.w) (DM+AC-20)], IV [DM+QB (100mg/kg b.w) (DM+QB-100)], V [DM+QB (250mg/kg b.w) (DM+QB-250)] and VI [DM+QB (500mg/kg b.w) (DM+QB-500)] groups. This study showed that the biochemical analysis showed a considerable increase in the HRDB, DB, LP, MDA and fluctuated ADSC in the II group as compared to that of control group whereas, AC and the plant lyophilized seed plant extract supplementations diet restored the STZ-induced diabetic complications towards the control. α-glycosidase activity in DM group showed statistically significant increase with respect to control group in small intestine. Moreover, in accordance with the effects of seed extract; in diabetic rat groups to whom acorn seed extract and acarbose were given, the levels of almost all the concerned parameters were reached to the ones measured at control group. As a result, it was concluded that acorn seed extract had certain healing effects on many complications caused by diabetes. [Display omitted]