The deleterious effects of smoking in bone mineralization and fibrillar matrix composition

• Published in: Life sciences (1973) Vol. 241; p. 117132
• Main Authors: Barbosa, Alexandre Póvoa, Lourenço, Juliana Dias, Junqueira, Jader Joel Machado, Larissa Emidio de França, Silva, Martins, Janaina S., Oliveira Junior, Manoel Carneiro, Begalli, Isadora, Velosa, Ana Paula Pereira, Olivo, Clarice Rosa, Bastos, Thiago Bernardes, Jorgetti, Vanda, Rodolfo de Paula, Vieira, Teodoro, Walcy Rosolia, Lopes, Fernanda D.T.Q.S.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Inc 15.01.2020
• Subjects: Animals; Bone remodeling; Calcification, Physiologic - drug effects; Cigarette Smoking - adverse effects; Collagen; Collagen Type I - metabolism; Cytokines - metabolism; Fracture healing; Male; Mice; Mice, Inbred C57BL; Osteogenesis - drug effects; Smoke - adverse effects; Smoking; Tibia - drug effects; Tibia - injuries; Tibia - metabolism; Tibia - pathology; Tibial Fractures - etiology; Tibial Fractures - metabolism; Tibial Fractures - pathology; Fracture healing; Bone remodeling; Collagen; Smoking
• ISSN: 0024-3205; 1879-0631
• DOI: 10.1016/j.lfs.2019.117132

This study aimed to verify the effects of cigarette smoke exposure in bone mineralization and fibrillar matrix composition as well as in bone healing after tibial fracture induction. C57Bl/6 Mice were assigned according to exposure and surgery: C room air; F room air and tibia open osteotomy; CS cigarette smoke; FCS cigarette smoke and tibia open osteotomy. In order to study fracture healing we performed, under anesthesia, a bone injury through a tibial shaft osteotomy. Bone samples were obtained to evaluate bone histomorphometry, trabecular morphology and volume, trabecular collagen types composition and presence of inflammatory cytokines and growth factors. CS exposure significantly reduced the thickness of bone trabeculae associated with decrease in mineralizing surface and mineral deposition rate, leading a lower bone formation rate and longer mineralization time. Resorption surface and osteoclastic surface were greater in the CS group, attesting increased resorptive action. There was a decrease in type I collagen deposition and genes expression in the CS and FCS groups compared to C group and in contrast there was an increase in type V collagen deposition and genes expression in the CS, FC and FSC groups compared to C group. Also, CS exposure induced a decrease in bone forming cytokines and an increase in inflammatory associated cytokines, and these changes were intensified under fracture conditions. Cigarette smoke exposure alters bone matrix composition and worsens bone mineralization, leading to bone fragility by increasing collagen V synthesis and deposition and impairing collagen I fibril forming and assembling. And these deleterious effects contributed to the worsening in fracture healing after tibia osteotomy.