Histone Acetylation in Insect Chromosomes

Acetylation of histones takes place along the salivary gland chromosomes of Chironomus thummi when RNA synthesis is active. It can be observed but not measured quantitatively by autoradiography of chromosome squashes. The "fixatives" commonly used in preparing squashes of insect chromosome...

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Published inScience (American Association for the Advancement of Science) Vol. 159; no. 3812; pp. 314 - 316
Main Authors Allfrey, V. G., Pogo, B. G. T., Littau, V. C., Gershey, E. L., Mirsky, A. E.
Format Journal Article
LanguageEnglish
Published United States American Association for the Advancement of Science 19.01.1968
Subjects
Acetates - metabolism
Acetylation
Animals
Arginine
Autoradiography
Cattle
Cell Nucleus - analysis
Chromosomes
Chromosomes - metabolism
Diptera - metabolism
Electrophoresis
Ethanol
Histones
Histones - metabolism
Hydrochloric Acid
Insect genetics
Liver
Liver - cytology
Rats
RNA
RNA - biosynthesis
Salivary glands
Salivary Glands - cytology
Squashes
Thymus Gland - cytology
Tritium
Uridine - metabolism
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Summary:Acetylation of histones takes place along the salivary gland chromosomes of Chironomus thummi when RNA synthesis is active. It can be observed but not measured quantitatively by autoradiography of chromosome squashes. The "fixatives" commonly used in preparing squashes of insect chromosomes preferentially extract the highly acetylated "arginine-rich" histone fractions; the use of such fixatives may explain the reported absence of histone acetylation in Drosophila melanogaster.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:0036-8075
1095-9203
DOI:10.1126/science.159.3812.314