Facile and green synthesis of chlorophyll-derived multi-color fluorescent carbonized polymer dots and their use for sensitive detection of hemin

• Published in: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy Vol. 310; p. 123841
• Main Authors: Liu, Tianjiao, Dong, Deming, Meng, Yingyi, Chen, Haijun, Liu, Chunyue, Qi, Zihan, Li, Anfeng, Ning, Yang
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 05.04.2024
• Subjects: Carbon; Carbonized polymer dots; Chlorophyll; Fluorescent Dyes; Hemin; Inner filter effect; Polymers; Quantum Dots; Spectrometry, Fluorescence - methods; White-emission; Chlorophyll; Inner filter effect; Hemin; White-emission; Carbonized polymer dots
• ISSN: 1386-1425; 1873-3557
• DOI: 10.1016/j.saa.2024.123841

[Display omitted] •The red, white and blue emissive carbonized polymer dots were successfully prepared from chlorophyll.•The Commission International d’Eclairage coordinates of white-emitted CPDs were located at (0.34, 0.32).•A sensitive fluorescence sensing system for determination of hemin has been developed on the basis of white-CPDs.•This system can achieve visual detection of hemin or blood sample basing on the proportional quenching phenomenon. Due to the very important role in physiological process, a simple and sensitive hemin detection method is necessarily required. Biomass-based carbonized polymer dots (CPDs) have been widely studied especially as fluorescence probe owing to the advantages of low toxicity and the variety of fluorescence color, yet there are still challenges in developing their multi-color emission property from the same raw materials. In this work, red, white and blue emissive CPDs derived from chlorophyll have been synthesized via hydrothermal method. Then white-emitted CPDs (white-CPDs) with the Commission International d’Eclairage (CIE) coordinates at (0.34, 0.32) were used to develop a fluorescence quenched sensing system for hemin determination. There is a good linear relationship between (F0-F)/F0 and concentration of hemin in the range of 0.1–0.95 μM with a detection limit of 0.043 μM, and the quenching mechanism was considered to be caused by inner filter effect (IFE). Moreover, it has been successfully used for hemin detection in serum and also for visual determination, which indicating great potential in applications of disease diagnoses and trace identification.