Metabolic and immune effects of immunotherapy with proinsulin peptide in human new-onset type 1 diabetes

• Published in: Science translational medicine Vol. 9; no. 402
• Main Authors: Alhadj Ali, Mohammad, Liu, Yuk-Fun, Arif, Sefina, Tatovic, Danijela, Shariff, Hina, Gibson, Vivienne B, Yusuf, Norkhairin, Baptista, Roman, Eichmann, Martin, Petrov, Nedyalko, Heck, Susanne, Yang, Jennie H M, Tree, Timothy I M, Pujol-Autonell, Irma, Yeo, Lorraine, Baumard, Lucas, Stenson, Rachel, Howell, Alex, Clark, Alison, Boult, Zoe, Powrie, Jake, Adams, Laura, Wong, Florence S, Luzio, Stephen, Dunseath, Gareth, Green, Kate, O'Keefe, Alison, Bayly, Graham, Thorogood, Natasha, Andrews, Robert, Leech, Nicola, Joseph, Frank, Nair, Sunil, Seal, Susan, Cheung, HoYee, Beam, Craig, Hills, Robert, Peakman, Mark, Dayan, Colin M
• Format: Journal Article
• Language: English
• Published: United States 09.08.2017
• Subjects: Adolescent; Adult; Autoantibodies - immunology; Autoantigens - immunology; C-Peptide - metabolism; Diabetes Mellitus, Type 1 - metabolism; Diabetes Mellitus, Type 1 - therapy; Double-Blind Method; Female; Humans; Immunophenotyping; Immunotherapy - methods; Male; Middle Aged; Peptides - therapeutic use; Proinsulin - therapeutic use; T-Lymphocytes, Regulatory - metabolism; Young Adult
• ISSN: 1946-6242
• DOI: 10.1126/scitranslmed.aaf7779

Immunotherapy using short immunogenic peptides of disease-related autoantigens restores immune tolerance in preclinical disease models. We studied safety and mechanistic effects of injecting human leukocyte antigen-DR4( )-restricted immunodominant proinsulin peptide intradermally every 2 or 4 weeks for 6 months in newly diagnosed type 1 diabetes patients. Treatment was well tolerated with no systemic or local hypersensitivity. Placebo subjects showed a significant decline in stimulated C-peptide (measuring insulin reserve) at 3, 6, 9, and 12 months versus baseline, whereas no significant change was seen in the 4-weekly peptide group at these time points or the 2-weekly group at 3, 6, and 9 months. The placebo group's daily insulin use increased by 50% over 12 months but remained unchanged in the intervention groups. C-peptide retention in treated subjects was associated with proinsulin-stimulated interleukin-10 production, increased FoxP3 expression by regulatory T cells, low baseline levels of activated β cell-specific CD8 T cells, and favorable β cell stress markers (proinsulin/C-peptide ratio). Thus, proinsulin peptide immunotherapy is safe, does not accelerate decline in β cell function, and is associated with antigen-specific and nonspecific immune modulation.