Molecular cloning and characterization of class I NF-κB transcription factor from pearl oyster (Pinctada fucata)

• Published in: Fish & shellfish immunology Vol. 33; no. 3; pp. 659 - 666
• Main Authors: Huang, Xian-De, Liu, Wen-Guang, Guan, Yun-Yan, Shi, Yu, Wang, Qi, Zhao, Mi, Wu, Shan-Zeng, He, Mao-Xian
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.09.2012
• Subjects: Amino Acid Sequence; Animals; Base Sequence; Class I NFκB transcription factor; DNA, Complementary - genetics; Gene cloning; Gene Expression Profiling; Gene Expression Regulation; Immunity response; Immunity, Innate; Molecular Sequence Data; NF-kappa B - chemistry; NF-kappa B - genetics; NF-kappa B - metabolism; Organ Specificity; Pearl oyster; Phylogeny; Pinctada - chemistry; Pinctada - genetics; Pinctada - immunology; Pinctada - microbiology; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger - metabolism; Sequence Alignment; Sequence Homology, Amino Acid; Vibrio alginolyticus - physiology; Pearl oyster; Gene cloning; Immunity response; Class I NFκB transcription factor
• ISSN: 1050-4648; 1095-9947
• DOI: 10.1016/j.fsi.2012.06.029

NF-κB transcription factors play central roles in many important physiological and pathological processes including innate immune responses. Here we report the cloning of an NF-κB transcription factor, PfRelish from pearl oyster Pinctada fucata, one of the most important bivalve mollusks for seawater pearl production. PfRelish full-length cDNA is 3916 bp with an open reading frame of 3558 bp encoding a putative protein of 1186 amino acids. The deduced PfRelish contains a N-terminal RHD, a nucleus localization signal, an IκB-like domain with six ankyrin repeats and a death domain at the C-terminus, which is similar to class I NF-κB transcription factors. Comparison and phylogenetic analysis revealed that class I NF-κBs in mollusks including PfRelish might have most distant relationship to the arthropod Relish. Further expression analysis showed that PfRelish was apparently upregulated after Vibrio alginolyticus injection, which suggested that PfRelish was involved in the immune response to V. alginolyticus. ► PfRelish from Pinctada fucata in mollusks was cloned. ► PfRelish might have most distant relationship to the arthropod Relish. ► PfRelish was involved in the immune response to Vibrio alginolyticus.