Increase of CD4(+)CD25(+) T cells in Smad3(-/-) mice

• Published in: World journal of gastroenterology : WJG Vol. 12; no. 15; pp. 2455 - 2458
• Main Authors: Wang, Zi-Bing, Cui, Yu-Fang, Liu, Yu-Qing, Jin, Wei, Xu, Han, Jiang, Zhu-Jun, Lu, Ya-Xin, Zhang, Ying, Liu, Xiao-Lan, Dong, Bo
• Format: Journal Article
• Language: English
• Published: United States Department of Immunology, Beijing Institute of Radiation Medicine, 27 Taiping Road, Beijing 100850, China%Third Affiliated Hospital of Xinxiang Medical College, Xinxiang 453003, Henan Province, China%Institute of Biotechnology, Beijing 100071, China 21.04.2006; Baishideng Publishing Group Co., Limited
• Subjects: Animals; CD4-Positive T-Lymphocytes - immunology; Inflammation - etiology; Leukocyte Count; Mice; Mice, Knockout; Neutrophils - immunology; Rapid Communication; Receptors, Interleukin-2 - metabolism; Smad3 Protein - deficiency; Smad3 Protein - genetics; Smad3 Protein - physiology; T-Lymphocyte Subsets - immunology; Transforming Growth Factor beta - metabolism; CD4+CD25+ T cells; Lymphocyte subpopulation; TGF-β signaling; SlMlAD3
• ISSN: 1007-9327; 2219-2840
• DOI: 10.3748/wjg.v12.i15.2455

To investigate the changes of lymphocyte subpopulations, especially CD4(+)CD25(+) T regulatory cells in Smad3(-/-) mice. Hematological changes and changes of lymphocyte subpopulations were detected in Smad3(-/-) mice using cell counter and flow cytometry, respectively, and compared to their littermate controls. The numbers of neutrophils and lymphocytes in peripheral blood were significantly increased in Smad3(-/-) mice compared to littermate controls. CD19(+) expressing cells in blood and spleen, and CD8(+) T cells in thymus were all markedly decreased in Smad3(-/-) mice. More important, Smad3(-/-) mice had an increased population of CD4(+)CD25(+) T cells in peripheral lymphoid tissues, including thymus, spleen, and lymph nodes. These observations suggest that the changes of lymphocyte subpopulations might play a role in susceptibility to inflammation of Smad3(-/-) mice.