Discrimination of live and dead cells with two different sets of signals and unique application in vivo imaging

• Published in: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy Vol. 231; p. 118115
• Main Authors: Wang, Weishan, Liu, Yong, Niu, Jie, Lin, Weiying
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 15.04.2020
• Subjects: Animals; Apoptosis; Apoptosiss; Cell Survival; Discriminating; Fluorescent Dyes - chemistry; HeLa Cells; Humans; Live and dead cells; Mice; Microscopy, Fluorescence; Normal and tumor mice; Optical Imaging; RAW 264.7 Cells; Zebrafish; Normal and tumor mice; Live and dead cells; Discriminating; Apoptosiss
• ISSN: 1386-1425; 1873-3557
• DOI: 10.1016/j.saa.2020.118115

Discriminating living and dead cells is of great significance for the study of apoptosis. In this work, we have developed a unique fluorescent probe (RPIC) for discriminating live and dead cells with duel-channel fluorescence imaging under double excitation and double emission mode. Dead cells treated with RPIC shows weak fluorescence signals in red channel, however, strong fluorescence signals are appeared in red channel in live cells. Weak and strong green fluorescence signals present at live cells and dead cells, respectively. Moreover, RPIC can detect successfully apoptosis of cancer cells. For in-vivo imaging, RPIC can discriminate successfully live and dead zebrafish with the same method. More interestingly, it is found that RPIC possesses the ability of discriminating normal mice and tumor mice. Herein, the development of RPIC is described as the first paradigm of probes that could discriminate live and dead cells under double excitation and double emission mode. [Display omitted] •RPIC could discriminate live and dead cells by under double excitation and double emission mode.•RPIC can detect successfully apoptosis of cancer cells.•RPIC can discriminate successfully live and dead zebrafish.•It is found that RPIC possess the ability of discriminating normal mice and tumor mice.