Glycocyamine functionalized magnetic layered double hydroxides with multiple affinity sites for trace phosphopeptides enrichment

• Published in: Analytica chimica acta Vol. 1136; pp. 25 - 33
• Main Authors: Jiang, Dandan, Duan, Limei, Jia, Qiong, Liu, Jinghai
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.11.2020
• Subjects: Caseins; Glycine - analogs & derivatives; Glycocyamine; Humans; Hydroxides; Layered double hydroxide; Magnetic nanoparticle; Magnetic Phenomena; Mass spectrometry; Phosphopeptide enrichment; Phosphopeptides; Proteomics; Magnetic nanoparticle; Glycocyamine; Layered double hydroxide; Mass spectrometry; Phosphopeptide enrichment
• ISSN: 0003-2670; 1873-4324
• DOI: 10.1016/j.aca.2020.07.057

Efficient enrichment and identification of phosphopeptides are of great significance in biological applications. Glycocyamine functionalized magnetic layered double hydroxides (Fe3O4@LDH@NH2-GAA) was fabricated through an easy process. The magnetic composite possessed high surface area, good biocompatibility, and fast magnetic response. Fe3O4@LDH@NH2-GAA, combining not only metal ions (Cu2+ and Ga3+) in LDH but also functional guanidyl groups in glycocyamine, offered multiple affinity sites for phosphopeptides enrichment. With these favorable characters, it exhibited high selectivity (β-casein: bovine serum albumin = 1:5000), low detection limit (0.1 fmol), satisfactory enrichment recovery (94.5%), high adsorption capacity (82.4 mg g−1), and good repetitiveness. Moreover, the efficient enrichment of phosphopeptides by Fe3O4@LDH@NH2-GAA from nonfat milk, human saliva, serum, and A549 cell lysates further confirmed its great potential for trace biological detection and proteomic analysis. In this study, a novel glycocyamine functionalized magnetic LDHs-based enrichment platform was designed. The developed material was employed as the adsorbent for the selective enrichment of phosphopeptides by coupling with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The material was successfully applied to enrich phosphopetides from standard peptide mixtures, nonfat milk, human saliva, serum, and A549 cell lysates. [Display omitted] •Glycocyamine functionalized magnetic layered double hydroxides was fabricated through an easy process.•The prepared material was applied to enrich phosphopeptides coupled with MALDI-TOF MS determinations.•The prepared material exhibited high sensitivity, selectivity, and good reusability for phosphopeptides enrichment.