Differential inhibition of Sphaeropsis sapinea morphotypes by a phenolic compound and several monoterpenes of red pine

• Published in: Phytopathology Vol. 87; no. 6; pp. 606 - 609
• Main Authors: Blodgett, J.T. (University of Wisconsin-Madison, Madison, WI.), Stanosz, G.R
• Format: Journal Article
• Language: English
• Published: St. Paul, MN American Phytopathological Society 01.06.1997
• Subjects: AGENT PATHOGENE; ALPHA-PINENE; ANTIFUNGAL PROPERTIES; BETA-PINENE; Biological and medical sciences; CHEMICAL COMPOSITION; COMPOSE PHENOLIQUE; COMPOSICION QUIMICA; COMPOSITION CHIMIQUE; COMPUESTOS FENOLICOS; CRECIMIENTO; CROISSANCE; DEVELOPMENTAL STAGES; DISEASE RESISTANCE; DOSAGE EFFECTS; ENFERMEDADES FUNGOSAS; ESPORAS; ETAPAS DE DESARROLLO; Fundamental and applied biological sciences. Psychology; FUNGAL DISEASES; Fungal plant pathogens; GAMMA-3-CARENE; GERMINACION; GERMINATION; GROWTH; INHIBICION; INHIBITION; MALADIE FONGIQUE; MICELIO; MINNESOTA; MONOTERPENE; MONOTERPENES; MONOTERPENOS; MYCELIUM; ORGANISMOS PATOGENOS; PATHOGENICITY; PATHOGENS; Pathology, epidemiology, host-fungus relationships. Damages, economic importance; PATHOTYPE; PATHOTYPES; PATOGENICIDAD; PATOTIPOS; PHENOLIC COMPOUNDS; Phytopathology. Animal pests. Plant and forest protection; PINOSYLVIN; PINUS BANKSIANA; PINUS RESINOSA; POUVOIR PATHOGENE; PROPIEDADES ANTIMICOSICAS; PROPRIETE ANTIFONGIQUE; RESISTANCE AUX MALADIES; RESISTENCIA A LA ENFERMEDAD; SPHAEROPSIS; SPORE; SPORES; STADE DE DEVELOPPEMENT; TANINOS; TANNIN; TANNINS; WISCONSIN; Pinus resinosa; Plant pathogen; Host agent relation; In vitro; Fungi; Antifungal agent; Softwood forest tree; Gymnospermae; Phenols; Monoterpene; Coniferales; Spermatophyta; Fungi Imperfecti; Thallophyta
• ISSN: 0031-949X; 1943-7684
• DOI: 10.1094/PHYTO.1997.87.6.606

The in vitro effects of a red pine phenolic compound (pinosylvin), a phenolic compound common to other species (tannic acid), and the major red pine monoterpenes (alpha-pinene, beta-pinene, and delta-3-carene) on spore germination and mycelial growth of Sphaeropsis sapinea were examined. Two A and two B morphotype isolates were used. At 88 microgram/mm2, pinosylvin inhibited spore germination of all four isolates (98 to 100%). At 8.8 microgram/mm2, spore germination of B isolates was inhibited more than that of A isolates (73 versus 30%). Pinosylvin also inhibited mycelial growth of B isolates more than that of A isolates (84 versus 13% at 88 microgram/mm2). Tannic acid stimulated or had little affect on spore germination and had little affect on mycelial growth of either morphotype. Spore germination of B isolates was inhibited more than that of A isolates by beta-pinene at saturation (79 versus 37%). Spore germination of B isolates was inhibited and germination of A isolates was stimulated by delta-3-carene below saturation (49 versus -7%). Mycelial growth of B isolates was inhibited more than that of A isolates by all monoterpenes at saturation. Differences observed between morphotypes below saturation were significant only for beta-pinene. These results demonstrate the biological activity of a phenolic compound and monoterpenes that occur in red pine. The differential responses might provide means of distinguishing morphotypes and offer a potential explanation for ecological specialization