Whole genome resequencing reveal patterns of genetic variation within Colletotrichum acutatum species complex from rubber trees in China

• Published in: Fungal genetics and biology Vol. 167; p. 103801
• Main Authors: Liu, Xianbao, Li, Boxun, Cai, Jimiao, Shi, Tao, Yang, Yang, Feng, Yanli, Huang, Guixiu
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.06.2023
• Subjects: China; Colletotrichum - genetics; Colletotrichum acutatum; Genetic Variation; Genomic recombination; Hevea - genetics; Multi-locus phylogenetic analysis; Pathogenicity profiling; Phylogeny; Plant Diseases; Whole-genome re-sequencing; China; Genomic recombination; Whole-genome re-sequencing; Pathogenicity profiling; Multi-locus phylogenetic analysis; Colletotrichum acutatum
• ISSN: 1087-1845; 1096-0937
• DOI: 10.1016/j.fgb.2023.103801

•A weak phylogeographic sub-structure was observed within Colletotrichum acutatum species complex from rubber trees in China.•Genetic recombination is main patterns of genetic variation within Colletotrichum acutatum species complex from rubber trees in China.•Morphological characters and levels of aggressiveness between populations show significant differences. The Colletotrichum acutatum species complex possesses a diverse number of important traits, such as a wide host range and host preference, different modes of reproduction, and different strategies of host infection. Research using comparative genomics has attempted to find correlations between these traits. Here, we used multi-locus techniques and gene genealogical concordance analysis to investigate the phylogenetic relationships and taxonomic status of the Colletotrichum acutatum species complex using field isolates obtained from rubber trees. The results revealed that the dominant species was C. australisinense, followed by C. bannaense, while strain YNJH17109 was identified as C. laticiphilum. The taxonomic status of strains YNLC510 and YNLC511 was undetermined. Using whole-genome single nucleotide polymorphism data to analyze population structure, 18 strains of C. australisinense were subsequently divided into four populations, one of which was derived from an admixture of two populations. In addition, the strains LD1687, GD1628, and YNLC516, did not belong to any populations, and were considered to be admixtures of two or more populations. A split decomposition network analysis also provided evidence for genetic recombination within Colletotrichum acutatum species complex from rubber trees in China. Overall, a weak phylogeographic sub-structure was observed. Analysis also revealed significant differences in morphological characters and levels of virulence between populations.