Specific fluorescence labeling of target proteins by using a ligand-4-azidophthalimide conjugate

We herein propose a simple affinity-labeling method using a ligand-4-azidophthalimide (AzPI) conjugate. As a proof of concept, we show that two different ligand-AzPI conjugates enabled highly specific fluorescence labeling of their individual target proteins even in crude cell lysates. This method w...

Full description

Saved in:
Bibliographic Details
Published inChemical communications (Cambridge, England) Vol. 53; no. 62; pp. 8751 - 8754
Main Authors Chiba, Kosuke, Asanuma, Miwako, Ishikawa, Minoru, Hashimoto, Yuichi, Dodo, Kosuke, Sodeoka, Mikiko, Yamaguchi, Takao
Format Journal Article
LanguageEnglish
Published England 2017
Online AccessGet full text

Cover

More Information
Summary:We herein propose a simple affinity-labeling method using a ligand-4-azidophthalimide (AzPI) conjugate. As a proof of concept, we show that two different ligand-AzPI conjugates enabled highly specific fluorescence labeling of their individual target proteins even in crude cell lysates. This method was also applied to label endogenous target proteins inside living cells.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1359-7345
1364-548X
DOI:10.1039/c7cc03252h