Application of chloroplast promoters of Cyanidioschyzon merolae for exogenous protein expression

• Published in: Algae (Korean Phycological Society) Vol. 33; no. 4; pp. 351 - 358
• Main Authors: Krupnik, Tomasz, Wasilewska, Wioleta, Drożak, Anna, Romanowska, Elżbieta, Zienkiewicz, Maksymilian
• Format: Journal Article
• Language: English
• Published: Seoul Korean Society of Phycology (Han'gug Joryu Haghoe) 01.12.2018; 한국조류학회I
• Subjects: Acetyltransferase; Antibiotics; Cell cycle; Chloramphenicol; Chloramphenicol O-acetyltransferase; Chloromycetin; Chloroplasts; Cyanidioschyzon merolae; Deoxyribonucleic acid; DNA; DNA polymerase; E coli; Gene expression; Gene sequencing; Genetic modification; Genetic transformation; Genomes; Industrial plants; Laboratories; Nucleotide sequence; Organic chemistry; Physiology; Plasmids; Plastic foam; Polyethylene glycol; Promoters; Protein biosynthesis; Protein synthesis; Proteins; Transcription; 생물학; United States > US
• ISSN: 1226-2617; 2093-0860
• DOI: 10.4490/algae.2018.33.12.5

The ability to transform the chloroplast of Cyanidioschyzon merolae was limited by lack of confirmed and reliable promoter sequences (among other reasons), capable of delivering stable or modulated DNA transcription followed by protein synthesis. Our research has confirmed the applicability of three selected chloroplast promoters in C. merolae chloroplast overexpression of the exogenous protein (i.e., chloramphenicol acetyltransferase) and genetic transformation. These results might facilitate further research on genetically modified strains of C. merolae to envisage yet unknown aspect of cellular and plastic physiology as well as C. merolae potential applications as bio-factories or sources of useful chemicals.