Interaction of the chlorite-based drug WF10 and chlorite with hemoglobin, methemoglobin and ferryl hemoglobin

• Published in: Archives of biochemistry and biophysics Vol. 585; pp. 82 - 89
• Main Authors: Pichert, Annelie, Arnhold, Jürgen
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.11.2015
• Subjects: Cells, Cultured; Chlorides - chemistry; Chlorine - chemistry; Chlorite; Erythrocytes - chemistry; Ferryl hemoglobin; Heme - chemistry; Hemoglobins - antagonists & inhibitors; Hemoglobins - chemistry; Hemolysis; Humans; Hydrogen Peroxide - chemistry; Kinetics; Methemoglobin; Methemoglobin - chemistry; Oxidation-Reduction; Oxides - chemistry; Oxyhemoglobin; Spectrophotometry; WF10; Hemolysis; Methemoglobin; Chlorite; Oxyhemoglobin; Ferryl hemoglobin; WF10
• ISSN: 0003-9861; 1096-0384
• DOI: 10.1016/j.abb.2015.09.009

The interaction of the chlorite-based drug solution WF10 with human oxyhemoglobin and oxidized hemoglobin forms was investigated monitoring the corresponding spectral changes in heme states. The chlorite component of WF10 converts oxyhemoglobin into methemoglobin with a rate of 35.4 M−1s−1. Methemoglobin is also formed upon the interaction of ferryl hemoglobin and WF10/chlorite. The rate of this interconversion depends on the oxidation state of ferryl hemoglobin. This rate is 114 M−1s−1, when ferryl hemoglobin was generated upon reaction of oxyhemoglobin and hydrogen peroxide. A considerable higher rate (6600 M−1s−1) is measured between the chlorite components of WF10 and ferryl hemoglobin after formation of the latter species from methemoglobin. WF10/chlorite inactivates also methemoglobin as evidenced by the continuous decrease of the Soret band and all other absorbances with a rate of 8.3 M−1s−1. In all interconversions, the chlorite component of WF10 was the active principle as shown in experiments applying pure chlorite at the same concentration as in WF10. Thus, WF10 is able to diminish efficiently the yield of cytotoxic hemoglobin species that might appear after excessive hemolysis of red blood cells under pathologic situations. [Display omitted] •The chlorite-containing drug WF10 converts oxyhemoglobin into methemoglobin.•WF10 (chlorite) inactivates methemoglobin.•Ferryl hemoglobin is converted by WF10 into methemoglobin.•Chlorite is the active principle of WF10 in all interconversions of hemoglobin forms.•Chlorite caused the same spectral changes applying a chlorite concentration as in WF10.