Fullerol-fluorescein isothiocyanate-concanavalin agglutinin phosphorescent sensor for the detection of alpha-fetoprotein and forecast of human diseases

• Published in: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy Vol. 115; pp. 136 - 144
• Main Authors: Liu, Jia-ming, Lin, Li-ping, Jiang, Shu-Lian, Cui, Ma Lin, Jiao, Li, Zhang, Xiao Yang, Zhang, Li-hong, Zheng, Zhi Yong, Lin, Xuan, Lin, Shao-qin
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 01.11.2013
• Subjects: Alpha-fetoprotein variant; alpha-Fetoproteins - analysis; Aniline Compounds; Biosensing Techniques; Concanavalin A - chemistry; Disease; Fluorescein-5-isothiocyanate - analogs & derivatives; Fluorescein-5-isothiocyanate - chemistry; Forecast and diagnosis of human diseases; Fullerenes - chemistry; Fullerol-fluorescein isothiocyanate; Humans; Humidity; Indicators and Reagents; Limit of Detection; Luminescence; Oxygen - chemistry; Phosphorescent labeling reagent; Phosphorescent sensor; Temperature; Time Factors; Phosphorescent sensor; Alpha-fetoprotein variant; Forecast and diagnosis of human diseases; Fullerol-fluorescein isothiocyanate; Phosphorescent labeling reagent
• ISSN: 1386-1425; 1873-3557
• DOI: 10.1016/j.saa.2013.06.031

A new phosphorescent sensor for the determination of alpha-fetoprotein variant (AFP-V) and the technical method for the forecast of human diseases have been developed based on the specific affinity adsorption reaction between –COOH of DMA-F-FITC-Con A and –NH2 in AFP-V. This study opened up the analytical application of sensor, F and lectin, but also provided a new way for the development of SSRTP. [Display omitted] •Fullerol-fluorescein isothiocyanate and N,N-dimethylaniline (F-FITC-DMA) was exploited.•Phosphorescent sensor has been designed using reaction between F-FITC-DMA and Con A.•The sensor could be applied to the detection of AFP-V and the forecast of diseases.•Mechanisms of labeling Con A and the determination of AFP-V were discussed.•The coupling technique of sensor, labeling method and phosphorimetry was developed. Based on the reaction of the active –OH group in fullerol (F) with the dissociated –COOH group in fluorescein isothiocyanate (FITC) to form an F-FITC and the enhanced effect of N, N-dimethylaniline (DMA) on phosphorescence signal of F-FITC, a new phosphorescent labeling reagent (DMA-F-FITC) was developed. What’s more, a phosphorescent sensor for the determination of alpha-fetoprotein variant (AFP-V) has been designed via the coupling technique of the high sensitivity for affinity adsorption–solid substrate-room temperature phosphorimetry (AA-SS-RTP) with the strong specificity reaction between DMA-F-FITC-Con A and AFP-V. The DMA-F-FITC increased the number of luminescent molecules in the biological target which improved the sensitivity of phosphorescent sensor. The proposed sensor was responsive, simple, selective and sensitive, and it has been applied to the determination of trace AFP-V in human serum and the forecast of human diseases using phosphorescence emission wavelength of F or FITC, with the results agreed well with those obtained by enzyme-linked immunoassay (ELISA). Meanwhile, the mechanisms for the labeling reaction and the sensing detection of AFP-V were discussed.