Laser speckle contrast imaging and Oxygen to See for assessing microcirculatory liver blood flow changes following different volumes of hepatectomy

• Published in: Microvascular research Vol. 110; pp. 14 - 23
• Main Authors: Li, Chong Hui, Ge, Xin Lan, Pan, Ke, Wang, Peng Fei, Su, Yi Nan, Zhang, Ai Qun
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.03.2017
• Subjects: Animals; Biomarkers - blood; Blood Flow Velocity; Cytokines - genetics; Cytokines - metabolism; Hepatectomy; Hepatectomy - adverse effects; Hepatectomy - methods; Inflammation Mediators - metabolism; Laser speckle contrast image; Laser-Doppler Flowmetry; Liver - blood supply; Liver - surgery; Liver Circulation; Liver Regeneration; Male; Microcirculation; Microcirculatory liver blood flow; Organ Size; Oxygen - blood; Oxygen to See (O2C); Perfusion Imaging - methods; Postoperative Complications - blood; Postoperative Complications - diagnosis; Postoperative Complications - genetics; Postoperative Complications - physiopathology; Predictive Value of Tests; Rats, Wistar; Regional Blood Flow; Spectrophotometry; Time Factors; Microcirculatory liver blood flow; Oxygen to See (O2C); Laser speckle contrast image; Hepatectomy
• ISSN: 0026-2862; 1095-9319
• DOI: 10.1016/j.mvr.2016.11.004

Portal hyperperfusion after extended hepatectomy or small-for-size liver transplantation may induce organ dysfunction and failure. This study was designed to monitor and characterize the hepatic microcirculatory perfusion following different volumes of hepatectomy in rats by using laser speckle contrast image (LSCI) and Oxygen to See (O2C), a spectrometric device. The microcirculatory liver blood flow of the rats that underwent 68%, 85% and 90% hepatectomy (68PH, 85PH and 90PH) was monitored with LSCI and O2C before and following the hepatectomy. The portal venous flow (PVF) and hepatic arterial flow (HAF) were measured with an ultrasonic flowmeter. Liver regeneration, liver injury, histologic evaluation and gene expression were also assessed at 12h, 24h, 3d and 7d post hepatectomy. All the 68PH and 85PH rats survived, and 57% of the 90PH rats survived. After hepatectomy, both PVF and HAF decreased transiently, with the PVF of the 85PH and 90PH rats significantly lower than that of the 68PH rats. In contrast, the PVF and HAF per gram of liver weight were greatly increased after liver resection and were proportional to the volume of resected liver. Correspondingly, the microcirculatory liver blood flow of the 68PH, 85PH and 90PH rats, as assessed by both LSCI and O2C, were increased after hepatectomy, and the 90PH group was significantly higher than the 68PH and 85PH groups. The hyperperfusion continued for approximately 3days and returned to baseline following the completion of liver regeneration. The liver venous oxygen saturation of the three groups decreased immediately after hepatectomy and returned to baseline from 24h after hepatectomy. The 90PH rats also showed delayed liver regeneration and the most severe liver injury, as reflected by increased serum ALT, AST and TBIL levels, hepatocellular vacuolization, and inflammatory and endothelial constriction gene expressions (TNF-α, IL-1β, MIP-1α, ET-1 and TM-1). Hepatic microcirculation hyperperfusion resulting from major and extended liver resection could be assessed by LSCI and O2C methods. The 90PH in rats led to extraordinary sinusoidal hyperperfusion, severe endothelial injury and liver failure. Monitoring the changes of hepatic microcirculation perfusion following extended hepatectomy or small-for-size liver transplantation may help to analyze the extent of hyperperfusion. •First use of LSCI and O2C for assessing hepatectomy-induced changes of liver microLBF•Both LSCI and O2C could be used to monitor the excessive increase of microLBF after hepatectomy.•Liver venous oxygen saturation decreased transiently after 68%, 85% and 90% hepatectomy.•Extended hepatectomy also led to severe liver injury, delayed liver regeneration and liver failure.