Bioreactor Analysis for the Corn-Cob Valorization in the Xylanase Production

• Published in: Waste and biomass valorization Vol. 9; no. 6; pp. 995 - 1001
• Main Authors: Charles-Rodríguez, Verónica, Guerrero-Mata, Alejandro, Martínez-Vázquez, Gabriela, Cruz-Hernández, Mario A., Belmares-Cerda, Ruth E., Robledo, Armando
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.06.2018; Springer Nature B.V
• Subjects: Agricultural production; Agricultural wastes; Biological models (mathematics); Biomass; Bioreactors; Cell culture; Computer simulation; Corn; Engineering; Environment; Environmental Engineering/Biotechnology; Enzymatic activity; Enzyme activity; Enzymes; Fermentation; Growth rate; Industrial Pollution Prevention; Industrial wastes; Kinetics; Mathematical analysis; Mathematical models; Optimization; Original Paper; Renewable and Green Energy; Solid state fermentation; Spores; Substrates; Waste Management/Waste Technology; Xylanase; Tray bioreactor; Mathematical modeling; Column bioreactor; Xylanase; Rhizomucor pusillus; Corn-cob
• ISSN: 1877-2641; 1877-265X
• DOI: 10.1007/s12649-016-9754-3

Purpose To achieve the optimization of the solid-state fermentation (SSF) processes where agro-industrial wastes are used, a better understanding of biological aspects (mathematical modeling) are needed, due the heterogeneity of these materials. The purpose of this study was to compare the effect of the bioreactor arrangement (Tray and Column) in the synthesis of xylanase by Rhizomucor pusillus strain in SSF process. Methods Fermentation process was realized in both, tray and column bioreactors, using corn-cob (CC) as a carrier-substrate. Tray bioreactor and packed-bed bioreactor, containing fungal spores, 3 g of CC as substrate and support, and 8 mL of minimum Czapek-Dox culture medium; were incubated at 50 °C for 5 days. Maximal specific growth rate ( μ max ), maximal biomass level ( X max ), product/biomass yield ( α ) and secondary coefficient of product formation or destruction ( β ) were estimated by mathematical models. Results High value of xylanase enzyme activity (587.6 U/gds) was found in the column bioreactor. The logistic equation for cell growth and Luedeking-Piret equation for xylanase production could satisfactorily simulate the bioreactors’ kinetics data. Conclusions The production of xylanase by Rhizomucor pusillus SOC-4A is affected by the type of bioreactor. Data showed that the most efficient bioreactor in production of the xylanase enzyme is the column bioreactor.