Alginate Hydrogel Beads for Immobilizing Single Photosynthetic Cells

Hydrogel beads of size 25-µm for immobilizing single photosynthetic cells of size approximately 5 µm were prepared by electrospraying and were characterized electrochemically using a microelectrode. A parametric study was then conducted on the size of the hydrogel beads containing the cells. In orde...

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Bibliographic Details
Published inInternational journal of precision engineering and manufacturing Vol. 21; no. 4; pp. 739 - 745
Main Authors Bae, Jonghyuk, Cho, Gu Young, Bai, Seoung Jai
Format Journal Article
LanguageEnglish
Published Seoul Korean Society for Precision Engineering 01.04.2020
Springer Nature B.V
Subjects
Alginates
Beads
Electrochemical analysis
Engineering
Hydrogels
Immobilization
Industrial and Production Engineering
Materials Science
Microelectrodes
Photosynthesis
Properties (attributes)
Reduction
Regular Paper
Immobilization
Microelectrode
Hydrogel bead
Electrochemical measurement
Electrospray
Photosynthetic cell
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Summary:Hydrogel beads of size 25-µm for immobilizing single photosynthetic cells of size approximately 5 µm were prepared by electrospraying and were characterized electrochemically using a microelectrode. A parametric study was then conducted on the size of the hydrogel beads containing the cells. In order to understand the electrochemical properties of the cells in the hydrogel bead, a microelectrode combined with a counter electrode was fabricated and characterized using microfabrication techniques; the electrochemical properties of the cells and beads were subsequently measured by chronoamperometry using this microelectrode system. The electrochemical properties of single cells immobilized in hydrogel were compared with those of single cells without immobilization by examining the oxygen-reduction currents for both cases. Changes in the oxygen-reduction current caused by photosynthesis in the cells show that an appropriate environment for cell survival is formed inside the hydrogel beads. We thus produced hydrogel beads containing photosynthetic cells that could be potentially useful for photosynthesis-based sensor applications.
ISSN:2234-7593
2005-4602
DOI:10.1007/s12541-019-00278-8