A deep eutectic solvent modified magnetic β-cyclodextrin particle for solid-phase extraction of trypsin

• Published in: Analytica chimica acta Vol. 1137; pp. 125 - 135
• Main Authors: Xu, Fangting, Wang, Yuzhi, Liu, Ziwei, Wei, Xiaoxiao, Chen, Jing, He, Xiyan, Li, Heqiong, Zhou, Yigang
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 15.11.2020
• Subjects: beta-Cyclodextrins; Deep eutectic solvent; Magnetic Phenomena; Magnetic solid phase extraction; Solid Phase Extraction; Solvents; Trypsin; Trypsin - isolation & purification; β-cyclodextrin; Trypsin; Deep eutectic solvent; Magnetic solid phase extraction; β-cyclodextrin
• ISSN: 0003-2670; 1873-4324
• DOI: 10.1016/j.aca.2020.09.005

An environmentally friendly deep eutectic solvent (DES) composed of trifluoroacetamide and benzyltrimethylammonium chloride modified β-cyclodextrin (CD) grafted magnetic beads (MB-NH2@CD@DES) were synthesized for the first time and applied to the solid-phase extraction of trypsin. Among the five trypsin extractants prepared in this work (MB, MB-NH2, MB-NH2@CD, MB-NH2@DES, MB-NH2@CD@DES), the extractant MB-NH2@CD@DES with higher extraction capacity for trypsin was selected as final extractant. The extraction capacity of MB-NH2@CD@DES for trypsin can reach up to 549.87 mg⋅g−1 under the optimized conditions. The Langmuir adsorption equilibrium was found fitted better with equilibrium relation between MB-NH2@CD@DES and trypsin than Freundlich adsorption equilibrium. And a superior extraction for trypsin was verified by comparing the extraction capacity of MB-NH2@CD@DES for trypsin and four other common proteins. Compared with some reported trypsin extractants, the MB-NH2@CD@DES had a shorter extraction process, higher extraction capacity, more convenient operation of separation, a safer and more environmentally friendly synthesis process. With the optimized eluent, a great elution rate (74.32%) of trypsin was achieved. The absolute recovery of trypsin in trypsin standard solution was calculated to be 16.8%. And the extraction capacity of MB-NH2@CD@DES toward trypsin still maintained well after ten times recycling and reuse. The detection limit (LOD) and quantitative limit (LOQ) were 0.072 mg⋅mL−1 and 0.240 mg⋅mL−1 respectively. By sodium dodecyl sulfate polyacrylamide gel electrophoresis experiment, the extraction ability of MB-NH2@CD@DES to trypsin from real sample was fully demonstrated. All above results showed the potential of fabricated MB-NH2@CD@DES as a superior extractant for trypsin from real complex samples. Schematic diagram of the preparation process of MB-NH2@CD@DES and its application in extraction of trypsin. [Display omitted] •A extractant modified with β-cyclodextrin and deep eutectic solvent was designed.•The extractant exhibited a superior extraction capacity for trypsin.•The solid phase extractant possessed a great reusability.•The extractant can extract trypsin from bovine pancreatic crude extract.