Active antioxidants in ex-vivo examination of burn wound healing by means of IR and Raman spectroscopies—Preliminary comparative research

• Published in: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy Vol. 173; pp. 924 - 930
• Main Authors: Pielesz, Anna, Biniaś, Dorota, Sarna, Ewa, Bobiński, Rafał, Kawecki, Marek, Glik, Justyna, Klama-Baryła, Agnieszka, Kitala, Diana, Łabuś, Wojciech, Paluch, Jadwiga, Kraut, Małgorzata
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 15.02.2017
• Subjects: Animals; Antioxidants - pharmacology; Burn wound healing; Burns - drug therapy; Burns - metabolism; Burns - pathology; Chickens; Humans; Hydrogels e.g. H4SiO4 × nH2O (5% and 7%); l-ascorbic acid; Skin - metabolism; Skin - pathology; Spectrophotometry, Infrared; Spectrum Analysis, Raman; Wound Healing - drug effects; l-ascorbic acid; Hydrogels e.g. H4SiO4×nH2O (5% and 7%); Burn wound healing; Hydrogels e.g. HSiO×nHO (5% and 7%)
• ISSN: 1386-1425; 1873-3557
• DOI: 10.1016/j.saa.2016.10.046

Being a complex traumatic event, burn injury also affects other organ systems apart from the skin. Wounds undergo various pathological changes which are accompanied by alterations in the molecular environment. Information about molecules may be obtained with the use of Raman spectroscopy and Fourier-transform infrared spectroscopy, and when combined, both methods are a powerful tool for providing material characterization. Alterations in the molecular environment may lead to identifying objective markers of acute wound healing. In general, incubation of samples in solutions of l-ascorbic acid and 5% and 7% orthosilicic acid organizes the collagen structure, whereas the increased intensity of the Raman bands in the region of 1500–800cm−1 reveals regeneration of the burn tissue. Since oxidative damage is one of the mechanisms responsible for local and distant pathophysiological events after burn, antioxidant therapy can prove to be beneficial in minimizing burn wounds, which was examined on the basis of human skin samples and chicken skin samples, the latter being subject to modification when heated to a temperature sufficient for the simulation of a burn incident. Scanning electron microscopic images of the surface of human burn injury skin samples (SA) and burn injury human skin incubated in the presence of l-ascorbic acid solution (SB) and H4SiO4×nH2O hydrogels (SC). [Display omitted] •Raman and FTIR spectroscopies for identifying of skin wound healing•Incubation of samples in solutions of l-ascorbic acid and orthosilicic acid organizes the collagen structure.•The increased intensity of the 1500–800cm−1 Raman bands reveals regeneration of the burn tissue.