Synthesis, characterization of α-amino acid Schiff base derived Ru/Pt complexes: Induces cytotoxicity in HepG2 cell via protein binding and ROS generation

• Published in: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy Vol. 163; pp. 1 - 7
• Main Authors: Alsalme, Ali, Laeeq, Sameen, Dwivedi, Sourabh, Khan, Mohd. Shahnawaz, Al Farhan, Khalid, Musarrat, Javed, Khan, Rais Ahmad
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 15.06.2016
• Subjects: Amino Acids - pharmacology; Cell Death - drug effects; Cell Survival - drug effects; Circular Dichroism; Coordination Complexes - chemical synthesis; Coordination Complexes - pharmacology; Hep G2 Cells; HSA binding; Humans; Inhibitory Concentration 50; Kinetics; Lysosomes - metabolism; Metal complexes; MTT assay; Neutral Red - metabolism; Platinum - pharmacology; Protein Binding - drug effects; Protein Structure, Secondary; Reactive Oxygen Species - metabolism; ROS generation; Ruthenium - pharmacology; Schiff Bases - chemical synthesis; Schiff Bases - pharmacology; Serum Albumin - chemistry; Serum Albumin - metabolism; Spectrometry, Fluorescence; ROS generation; Metal complexes; MTT assay; HSA binding
• ISSN: 1386-1425; 1873-3557
• DOI: 10.1016/j.saa.2016.03.012

We have synthesized two new complexes of platinum (1) and ruthenium (2) with α-amino acid, l-alanine, and 2,3-dihydroxybenzaldehyde derived Schiff base (L). The ligand and both complexes were characterized by using elemental analysis and several other spectroscopic techniques viz; IR, 1H, 13C NMR, EPR, and ESI-MS. Furthermore, the protein-binding ability of synthesized complexes was monitored by UV–visible, fluorescence and circular dichroism techniques with a model protein, human serum albumin (HSA). Both the PtL2 and RuL2 complexes displayed significant binding towards HSA. Also, in vitro cytotoxicity assay for both complexes was carried out on human hepatocellular carcinoma cancer (HepG2) cell line. The results showed concentration-dependent inhibition of cell viability. Moreover, the generation of reactive oxygen species was also evaluated, and results exhibited substantial role in cytotoxicity. [Display omitted] •Synthesis and characterization of platinum and ruthenium complexes•Binding studies of these complexes with human serum albumin•In vitro anticancer studies viz, MTT assay, ROS generation