Somatic organogenesis and plant regeneration in Ricinus communis

• Published in: Biologia plantarum Vol. 52; no. 1; pp. 17 - 25
• Main Authors: Ganesh Kumari, K.,Bharathidasan Univ., Tiruchirappalli (India). Dept. of Plant Science, Ganesan, M.,Bharathidasan Univ., Tiruchirappalli (India). Dept. of Plant Science, Jayabalan, N.,Bharathidasan Univ., Tiruchirappalli (India). Dept. of Plant Science
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.03.2008
• Subjects: 6-BENZILADENINA; 6-BENZYLADENINE; ACIDE AMINE; ACIDE GIBBERELLIQUE; ACIDE PANTOTHENIQUE; ACIDO GIBERELICO; ACIDO PANTOTENICO; ADDITIF; ADDITIVES; ADITIVOS; AIA; AMINO ACIDS; AMINOACIDOS; ANA; AUXINAS; AUXINE; AUXINS; Biomedical and Life Sciences; CAL; CALLO; CALLUS; CARBOHIDRATOS; CARBOHYDRATES; COTILEDONES; COTYLEDON; COTYLEDONS; CULTURE MEDIA; DURCISSEMENT; ENDURECIMIENTO; EXPLANT; EXPLANTES; EXPLANTS; GERMINACION; GERMINATION; GIBBERELLIC ACID; GLUCIDE; GLUTAMINA; GLUTAMINE; GRAINE; HARDENING; http://www.fao.org/aos/agrovoc#c_10204; http://www.fao.org/aos/agrovoc#c_118; http://www.fao.org/aos/agrovoc#c_1300; http://www.fao.org/aos/agrovoc#c_16185; http://www.fao.org/aos/agrovoc#c_1930; http://www.fao.org/aos/agrovoc#c_24136; http://www.fao.org/aos/agrovoc#c_24576; http://www.fao.org/aos/agrovoc#c_27520; http://www.fao.org/aos/agrovoc#c_27791; http://www.fao.org/aos/agrovoc#c_3160; http://www.fao.org/aos/agrovoc#c_3247; http://www.fao.org/aos/agrovoc#c_3292; http://www.fao.org/aos/agrovoc#c_34114; http://www.fao.org/aos/agrovoc#c_342; http://www.fao.org/aos/agrovoc#c_34350; http://www.fao.org/aos/agrovoc#c_3778; http://www.fao.org/aos/agrovoc#c_4102; http://www.fao.org/aos/agrovoc#c_5061; http://www.fao.org/aos/agrovoc#c_5187; http://www.fao.org/aos/agrovoc#c_5542; http://www.fao.org/aos/agrovoc#c_5968; http://www.fao.org/aos/agrovoc#c_6600; http://www.fao.org/aos/agrovoc#c_6651; http://www.fao.org/aos/agrovoc#c_6946; http://www.fao.org/aos/agrovoc#c_726; http://www.fao.org/aos/agrovoc#c_754; IAA; IN VITRO REGENERATION; KINETIN; KINETINE; Life Sciences; MEDIO DE CULTIVO; MICROPROPAGACION; MICROPROPAGATION; MILIEU DE CULTURE; NAA; NITRATE; NITRATES; NITRATOS; ORGANOGENESE; ORGANOGENESIS; Original Papers; PANTOTHENIC ACID; PLANT GROWTH SUBSTANCES; Plant Sciences; QUINETINA; RACINE; RAICES; REGENERACION IN VITRO; REGENERATION IN VITRO; RICINUS COMMUNIS; ROOTS; SEEDS; SEMILLA; SUBSTANCE DE CROISSANCE VEGETALE; SUSTANCIAS DE CRECIMIENTO VEGETAL; THIDIAZURON; TIDIAZURON; cytokinins; additives; auxins; callus cultures; amino acids; root induction; cotyledon explants
• ISSN: 0006-3134; 1573-8264
• DOI: 10.1007/s10535-008-0003-x

An in vitro propagation system was developed for Ricinus communis cv. TMV 6 through cotyledon derived callus cultures. The impact of different concentrations of auxins, cytokinins, additives, amino acids and sugars were evaluated for callus induction and shoot proliferation. Green compact nodular organogenic callus was obtained on the medium fortified with Murashige and Skoog (MS) salts, B5 vitamins, 2.0 mg/cubic dm 6-benzyladenine and 0.8 mg/cubic dm alpha-naphthalene acetic acid (NAA). Multiple shoot proliferation from the callus cultures was achieved on the medium with MS salts, B5 vitamins, 2.5 mg/cubic dm thidiazuron (TDZ), 0.4 mg/cubic dm NAA and 15 mg/cubic dm glutamine. During multiple shoot induction the phenolic secretion was controlled by the addition of 15 mg/cubic dm polyvinylpyrolidone. The proliferated shoots were elongated on the medium comprising MS salts, B5 vitamins, 1.5 mg/cubic dm TDZ and 0.3 mg/cubic dm gibberellic acid. The elongated shoots were rooted on the medium containing MS salts, B5 vitamins, 0.3 mg/cubic dm indole-3-butyric acid and 0.6 mg/cubic dm silver nitrate. After root induction, the plants were hardened in earthen pots containing sand, soil and vermiculite.