Self-assembled fluorescent Ce(Ⅲ) coordination polymer as ratiometric probe for HIV antigen detection

• Published in: Analytica chimica acta Vol. 1084; pp. 116 - 122
• Main Authors: Du, Mingyuan, Li, Ningxing, Mao, Guobin, Liu, Yucheng, Wang, Xinxin, Tian, Songbai, Hu, Qinxue, Ji, Xinghu, Liu, Yalan, He, Zhike
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 25.11.2019
• Subjects: Cerium - chemistry; Coordination Complexes - chemical synthesis; Coordination Complexes - chemistry; Coordination polymer; Fluorescent Dyes - chemical synthesis; Fluorescent Dyes - chemistry; HIV Antigens - analysis; HIV Infections - diagnostic imaging; Humans; p24 detection; Polymers - chemistry; Ratiometric probe; Self-assemble; p24 detection; Ratiometric probe; Coordination polymer; Self-assemble
• ISSN: 0003-2670; 1873-4324
• DOI: 10.1016/j.aca.2019.08.010

The viral capsid protein p24 of human immunodeficiency virus is expressed at different level during viral invasion. Detection of p24 is of great importance in acquired immunodeficiency syndrome monitoring and therapy. A ratiometric probe that is easily-synthesized was constructed based on self-assembled fluorescent Ce(Ⅲ) and fluorescein. Fluorescein was used as reference. Hydrogen peroxide quenches the fluorescence of the Ce(III) easily but does not quench the fluorescence of fluorescein. The mechanism of reaction was discussed. Benefiting from the sensitive response to hydrogen peroxide, this probe was applied for p24 detection in enzyme linked immunoassay. The fluorescence ratio was in a good linear relationship with the concentration of p24, and the detection limit was 1.1 pg mL−1. This proposed method has shown potential in virus detection with easy operation. [Display omitted] •Simple and effective preparation of fluorescent coordination polymer was proposed.•The ratiometric probe do not need chemical modification or complicated separation.•The ratiometric probe was applied in p24 detection, the detection limit was 1.1 pg mL−1.