Fast dopamine detection based on evanescent wave detection platform

• Published in: Analytica chimica acta Vol. 1191; p. 339312
• Main Authors: Pan, Lipeng, Zou, Mingye, Ma, Fangxing, Kong, Lingqing, Zhang, Changnan, Yang, Likun, Zhu, Anna, Long, Feng, Liu, Xiang-Yang, Lin, Naibo
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 25.01.2022
• Subjects: Dopamine; Electron Transport; Evanescent wave; Fluorescence sensor; Gold; Limit of Detection; Monophenol Monooxygenase - metabolism; Optical fiber; Spectrometry, Fluorescence; Dopamine; Fluorescence sensor; Evanescent wave; Optical fiber
• ISSN: 0003-2670; 1873-4324
• DOI: 10.1016/j.aca.2021.339312

A compact evanescent wave detection platform (EWDP) is developed for the detection of fluorescence gold nanoclusters. The EWDP employs a simple optical system and a Si-based photodetector SOP-1000 assembly to improve the optical efficiency and detection sensitivity. A microfluidic sample cell is also used to decrease the amount of analyte to 200 μL (The volume of sample cell is really about 30 μL). On this basis, we design a strategy for detecting dopamine (DA) based on the photoinduced electron transfer (PET) quenching mechanism. By introduction of tyrosinase (TYR) during the detection, the testing time is shortened to 1 min. The fluorescence emission signal decreased dramatically and the quenching ratio (F0–F)/F0 is linearly related to the concentration of DA in the range of 0.03–60 μM with a detection limit of 0.03 μM. Additionally, this detection platform has potential applications for DA fast detection in the microsamples. [Display omitted] •A novel platform is built for detection of dopamine (DA) based on evanescent wave.•The volume of sample for detection only needs 200 μL totally.•The reaction time shorten to 1 min under the catalysis of tyrosinase.•Conditions were optimized to get excellent comprehensive property and recoveries.•It was proved that the quenching was caused by photoinduced electron transfer.