The therapeutic effects and optimal timing of granulocyte colony stimulating factor intrauterine administration during IVF-ET

• Published in: Life sciences (1973) Vol. 317; p. 121444
• Main Authors: Won, Jieun, Lee, Danbi, Lee, Yu-Gyeong, Hong, Seon-Hwa, Kim, Jee Hyun, Kang, Youn-Jung
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Inc 15.03.2023
• Subjects: Animals; Embryo Implantation; Embryo Transfer - methods; Endometrial angio-genesis; Endometrial receptivity; Evidence-based standardized protocol; Female; Fertilization in Vitro - methods; Granulocyte Colony-Stimulating Factor; Mice; Non-invasive treatment; Pregnancy; Pregnancy Rate; Endometrial angio-genesis; Embryo implantation; Non-invasive treatment; Granulocyte colony stimulating factor; Evidence-based standardized protocol; Endometrial receptivity
• ISSN: 0024-3205; 1879-0631
• DOI: 10.1016/j.lfs.2023.121444

Most of embryos fail to produce live offspring during In Vitro Fertilization-Embryo Transfer (IVF-ET) procedure. There is a dearth of research activity addressing this problem despite the significant population of women suffering from repeated implantation failure after transfer of high-quality of embryos. As a clinically accessible option, granulocyte colony stimulating factor (G-CSF) is often used for the treatment to improve the rates of embryo implantation. However, there are currently no evidence-based standardized protocol for the clinical use of G-CSF. G-CSF was administered into one side of mouse uterine horns and saline was infused into the other side of horns as a control. Intrauterine G-CSF administration showed maximal effects 24 h after administration in enhancing endometrial receptivity and subsequent increase of angiogenesis by demonstrating elevated integrin β3 and OPN and reduced cytotoxicity of NK cells. Furthermore, G-CSF administration 24 h prior to embryo transfer promoted the stability of attached embryos at the early stage of implantation in vitro. Our findings suggest as new consensus criteria providing a potential therapeutic strategy of the clinical use of G-CSF to achieve maximal effects of IVF-ET for patients who are suffering from repeated implantation failure with the problems with endometrial receptivity.