Inhibition of precancerous lesions development in kidneys by chrysin via regulating hyperproliferation, inflammation and apoptosis at pre clinical stage

• Published in: Archives of biochemistry and biophysics Vol. 606; pp. 1 - 9
• Main Authors: Rashid, Summya, Nafees, Sana, Vafa, Abul, Afzal, Shekh Muhammad, Ali, Nemat, Rehman, Muneeb U., Hasan, Syed Kazim, Siddiqi, Aisha, Barnwal, Preeti, Majed, Ferial, Sultana, Sarwat
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 15.09.2016
• Subjects: Animals; Anticarcinogenic Agents - chemistry; Antioxidants - chemistry; Apoptosis; Carcinogenesis; Cell Proliferation; Chrysin; Ferric Compounds; Flavonoids - chemistry; Gene Expression Regulation, Neoplastic; Hyper proliferation; Inflammation; Kidney - pathology; Kidney Neoplasms - physiopathology; Kidney Neoplasms - prevention & control; Lipid Peroxidation; Male; Nitrilotriacetic Acid - analogs & derivatives; Oxidative Stress; Precancerous Conditions - chemically induced; Precancerous Conditions - physiopathology; Rats; Rats, Wistar; Renal cell carcinoma; ROS; Up-Regulation; NADPH; GSSG; DTNB; CH; Inflammation; CDNB; Hyper proliferation; GR; MDA; DEN; EDTA; GPx; BSA; LDH; TBA; Chrysin; Renal cell carcinoma; Fe-NTA; ROS; CAT; BUN; GSH; Apoptosis
• ISSN: 0003-9861; 1096-0384
• DOI: 10.1016/j.abb.2016.07.004

Chrysin (CH) is natural, biologically active compound, belongs to flavoniod family and possesses diverse pharmacological activities as anti-inflammatory, anti-oxidant and anti-cancer. It is found in many plants, honey and propolis. In the present study, we investigated the chemopreventive efficacy of CH against N-nitrosodiethylamine (DEN) initiated and Fe-NTA induced precancerous lesions and its role in regulating oxidative injury, hyperproliferation, tumor incidences, histopathological alterations, inflammation, and apoptosis in the kidneys of Wistar rats. Renal cancer was initiated by single intraperitoneal (i.p.) injection of DEN (200 mg/kg bw) and promoted by twice weekly injection of ferric nitrilotriacetate (Fe-NTA) 9 mg Fe/kg bw for 16 weeks. CH attenuated Fe-NTA enhanced renal lipid peroxidation, serum toxicity markers and restored renal anti oxidant armory significantly. CH supplementation suppressed the development of precancerous lesions via down regulation of cell proliferation marker like PCNA; inflammatory mediators like TNF-α, IL-6, NFkB, COX-2, iNOS; tumor incidences. CH up regulated intrinsic apoptotic pathway proteins like bax, caspase-9 and caspase-3 along with down regulation of Bcl-2 triggering apoptosis. Histopathological and ultra structural alterations further confirmed biochemical and immunohistochemical results. These results provide powerful evidence for the chemopreventive efficacy of CH against chemically induced renal carcinogenesis possibly by modulation of multiple molecular pathways. [Display omitted] •Up regulation of inflammatory marker proteins-mechanism behind renal cancer.•Increase in tumor incidences and proliferation-mechanism behind renal cancer.•Down regulation of anti-oxidant armory and apoptosis-mechanism behind renal cancer.•Damage in normal histological and cellular architecture.•Chrysin attenuates renal carcinogenesis by modulation of above molecular pathways.