Detection of glycoproteins B and H genotypes to predict the development of Cytomegalovirus disease in solid organ transplant recipients

• Published in: Journal of clinical virology Vol. 109; pp. 50 - 56
• Main Authors: Barrado, Laura, Prieto, Columbiana, Hernando, Susana, Folgueira, Lola
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.12.2018
• Subjects: CMV genotypes; Prognosis; Transplantation; Viral load; Transplantation; Prognosis; CMV genotypes; Viral load
• ISSN: 1386-6532; 1873-5967
• DOI: 10.1016/j.jcv.2018.11.001

•Mixed gB genotypes are associated with clinical manifestations and higher viral loads.•gB3 and gH1 genotypes are detected in patients who present with asymptomatic viremia.•The detection of gB and gH genotypes can help to predict CMV disease development. Our study focuses on the role that human Cytomegalovirus (CMV) genotypes play in the development of disease. (1) To analyze the frequency of various genotype envelope proteins (gB, gH) in a group of solid organ transplant (SOT) recipients; (2) to assess their correlation with CMV disease; (3) to study the association between any of the genotypes and viral loads. A retrospective observational study conducted by analyzing CMV gB and gH genotypes detected with real-time polymerase chain reaction (PCR)-specific assays in 162 CMV-positive blood samples from 62 SOT recipients. Demographic, clinical, and microbiological data were recorded. Mixed gB genotypes were associated with viral syndrome (70%, p =  .004), earlier presentation of symptoms (48.27 ± 27.03 versus 74.33 ± 47.25 days, respectively, p =  .001), and higher median of the plasma viral load log10 (UI/ml) than infection with a single genotype (p =  .004). Furthermore, the gB3 genotype was detected more frequently in patients who presented with asymptomatic viremia (77.27%, p <  .0001). The gH1 genotype was more frequent (65%) in patients who presented with asymptomatic viremia (p =  .003), and it caused infection later than gH2 or the mixed genotype (84.88 ± 48.10 versus 57.91 ± 39.18 days, respectively, p <  .001). Patients who presented mixed gB genotypes more frequently developed clinical manifestations and earlier, higher, plasma viral loads. The detection of gB and gH genotypes by real-time PCR can provide relevant information to stratify the risk of SOT recipients to develop symptomatic infection by CMV.