Membrane-associated NRPM proteins are novel suppressors of stomatal production in Arabidopsis

In Arabidopsis, stomatal development and patterning require tightly regulated cell division and cell-fate differentiation that are controlled by key transcription factors and signaling molecules. To identify new regulators of stomatal development, we assay the transcriptomes of plants bearing enrich...

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Published inCurrent biology Vol. 34; no. 4; pp. 881 - 894.e7
Main Authors Xue, Xueyi, Wang, Lu, Huang, Aobo, Liu, Zehao, Guo, Xiaoyu, Sang, Yuying, Zhu, Jian-Kang, Xue, Huiling, Dong, Juan
Format Journal Article
LanguageEnglish
Published England Elsevier Inc 26.02.2024
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Summary:In Arabidopsis, stomatal development and patterning require tightly regulated cell division and cell-fate differentiation that are controlled by key transcription factors and signaling molecules. To identify new regulators of stomatal development, we assay the transcriptomes of plants bearing enriched stomatal lineage cells that undergo active division. A member of the novel regulators at the plasma membrane (NRPM) family annotated as hydroxyproline-rich glycoproteins was identified to highly express in stomatal lineage cells. Overexpressing each of the four NRPM genes suppressed stomata formation, while the loss-of-function nrpm triple mutants generated severely overproduced stomata and abnormal patterning, mirroring those of the erecta receptor family and MAPKKK yoda null mutants. Manipulation of the subcellular localization of NRPM1 surprisingly revealed its regulatory roles as a peripheral membrane protein instead of a predicted cell wall protein. Further functional characterization suggests that NRPMs function downstream of the EPF1/2 peptide ligands and upstream of the YODA MAPK pathway. Genetic and cell biological analyses reveal that NRPM may promote the localization and function of the ERECTA receptor proteins at the cell surface. Therefore, we identify NRPM as a new class of signaling molecules at the plasma membrane to regulate many aspects of plant growth and development. [Display omitted] •NRPM1 is highly expressed during stomatal asymmetric cell division•NRPMs are four novel stomatal repressors functioning inside the plasma membrane•Mutating NRPM genes attenuates the plasma membrane localization of ERECTA•NRPMs function upstream of the MAPKKK YDA in stomatal development External signaling pathways heavily influence plant development in Arabidopsis. Xue et al. identify the NRPM proteins as novel signaling molecules at the plasma membrane that suppress stomatal production. This underscores an unrecognized regulatory module between the membrane receptors and the MAPK cascade.
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ISSN:0960-9822
1879-0445
DOI:10.1016/j.cub.2024.01.052