Fluorescence detection of single-nucleotide differences using aptamer-forming binary DNA probes

We report a simple method for fluorescence detection of single-nucleotide alterations in a long target DNA, which is based on the formation of a three-way-junction-structured cholic-acid-binding DNA aptamer by the hybridization of the target with binary DNA probes. The new method was successfully ex...

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Bibliographic Details
Published inAnalyst (London) Vol. 141; no. 21; pp. 6087 - 6092
Main Authors Kamoto, Saori, Hyuga, Masumi, Kato, Teru
Format Journal Article
LanguageEnglish
Published England 17.10.2016
Subjects
Alterations
Aptamers, Nucleotide
Cholic Acid - chemistry
Cytochrome P-450 CYP2C19 - genetics
Deoxyribonucleic acid
DNA - chemistry
DNA Probes
Fluorescence
Formations
Genes
Genotyping Techniques
Humans
Nucleic Acid Hybridization
Polymorphism, Single Nucleotide
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Summary:We report a simple method for fluorescence detection of single-nucleotide alterations in a long target DNA, which is based on the formation of a three-way-junction-structured cholic-acid-binding DNA aptamer by the hybridization of the target with binary DNA probes. The new method was successfully exploited for SNP genotyping of human CYP2C19 gene.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0003-2654
1364-5528
DOI:10.1039/c6an00912c