Nanopore sequencing: flourishing in its teenage years

• Published in: Journal of genetics and genomics Vol. 51; no. 12; pp. 1361 - 1374
• Main Authors: Zhang, Tianyuan, Li, Hanzhou, Jiang, Mian, Hou, Huiyu, Gao, Yunyun, Li, Yali, Wang, Fuhao, Wang, Jun, Peng, Kai, Liu, Yong-Xin
• Format: Journal Article
• Language: English
• Published: China Elsevier Ltd 01.12.2024
• Subjects: Accuracy; Application; Direct RNA sequencing; Genomics - methods; High-Throughput Nucleotide Sequencing - methods; Humans; Long-read sequencing; Metagenomics; Metagenomics - methods; Nanopore sequencing; Nanopore Sequencing - methods; Outlook; Sequence Analysis, DNA - methods; Direct RNA sequencing; Metagenomics; Accuracy; Nanopore sequencing; Outlook; Long-read sequencing; Application
• ISSN: 1673-8527
• DOI: 10.1016/j.jgg.2024.09.007

Over the past decade, nanopore sequencing has experienced significant advancements and changes, transitioning from an initially emerging technology to a significant instrument in the field of genomic sequencing. However, as advancements in next-generation sequencing technology persist, nanopore sequencing also improves. This paper reviews the developments, applications, and outlook on nanopore sequencing technology. Currently, nanopore sequencing supports both DNA and RNA sequencing, making it widely applicable in areas such as telomere-to-telomere (T2T) genome assembly, direct RNA sequencing (DRS), and metagenomics. The openness and versatility of nanopore sequencing have established it as a preferred option for an increasing number of research teams, signaling a transformative influence on life science research. As the nanopore sequencing technology advances, it provides a faster, more cost-effective approach with extended read lengths, demonstrating the significant potential for complex genome assembly, pathogen detection, environmental monitoring, and human disease research, offering a fresh perspective in sequencing technologies.