The expression and biological information analysis of miR-375-3p in head and neck squamous cell carcinoma based on 1825 samples from GEO, TCGA, and peer-reviewed publications

• Published in: Pathology, research and practice Vol. 214; no. 11; pp. 1835 - 1847
• Main Authors: Cen, Wei-ning, Pang, Jin-shu, Huang, Jia-cheng, Hou, Jia-yin, Bao, Wen-guang, He, Rong-quan, Ma, Jie, Peng, Zhi-gang, Hu, Xiao-hua, Ma, Fu-chao
• Format: Journal Article
• Language: English
• Published: Germany Elsevier GmbH 01.11.2018
• Subjects: Area Under Curve; Biological pathway; Clinical significance; Gene Expression Regulation, Neoplastic - genetics; HNSCC; Humans; MicroRNAs - biosynthesis; miR-375-3p; Prognosis; ROC Curve; Squamous Cell Carcinoma of Head and Neck - genetics; Squamous Cell Carcinoma of Head and Neck - mortality; Squamous Cell Carcinoma of Head and Neck - pathology; Transcriptome; Prognosis; Clinical significance; miR-375-3p; HNSCC; Biological pathway
• ISSN: 0344-0338; 1618-0631
• DOI: 10.1016/j.prp.2018.09.010

The specific expression level and clinical significance of miR-375-3p in HNSCC had not been fully stated, as well as the overall biological function and molecular mechanisms. Therefore, we purpose to carry out a comprehensive meta-analysis to further explore the clinical significance and potential function mechanism of miR-375-3p in HNSCC. HNSCC-related data was gained from Gene Expression Omnibus (GEO), The Cancer Genome Atlas (TCGA), and peer-reviewed journals. A meta-analysis was carried out to comprehensively explore the relationship between miR-375-3p expression level and clinicopathological features of HNSCC. And summary receiver operating characteristic (SROC) curve analysis was applied for evaluating disease diagnosis value of miR-375-3p. In addition, a biological pathway analysis was also performed to assess the possible molecular mechanism of miR-375-3p in HNSCC. A total of 24 available records and references were added into analysis. The overall pooled meta-analysis outcome revealed a relatively lower expression level of miR-375-3p in HNSCC specimens than that in non-cancerous controls (P < 0.001). And SROC curve analysis showed that the pooled area under the SROC curve (AUC) was 0.90 (95%CI: 0.88–0.93). In addition, biological pathway analysis indicated that LAMC1, EDIL3, FN1, VEGFA, IGF2BP2, and IGF2BP3 maybe the latent target genes of miR-375-3p, which were greatly enriched in the pathways of beta3 integrin cell surface interactions and the binding of RNA via the insulin-like growth factor-2 mRNA-binding protein (IGF2BPs/IMPs/VICKZs). MiR-375-3p expression clearly decreased in HNSCC samples compared with non-cancerous controls. Meanwhile, miR-375-3p may serve as a tumor suppressor via regulating tumor-related genes LAMC1, EDIL3, FN1, VEGFA, IGF2BP2, and IGF2BP3 in HNSCC.