Determination of glycated albumin using a Prussian blue nanozyme-based boronate affinity sandwich assay

• Published in: Analytica chimica acta Vol. 1134; pp. 41 - 49
• Main Authors: Son, Seong Eun, Gupta, Pramod K., Hur, Won, Choi, Hyun, Lee, Han Been, Park, Yosep, Seong, Gi Hun
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 16.10.2020
• Subjects: Boronate affinity; Colorimetry; Ferrocyanides; Glycated albumin; Nanozyme; Peroxidase-like activity; Prussian blue nanoparticles; Sandwich assay; Serum Albumin; Prussian blue nanoparticles; Glycated albumin; Nanozyme; Peroxidase-like activity; Boronate affinity; Sandwich assay
• ISSN: 0003-2670; 1873-4324
• DOI: 10.1016/j.aca.2020.08.015

Nanozymes are effective substitutes for natural enzymes and offer multiple advantages. Here, synthesized Prussian blue nanoparticles (PBNPs) exhibited excellent peroxidase-like activity, catalyzing the oxidation of 3,5,3′,5′-tetramethylbenzidine (TMB) in the presence of hydrogen peroxide within 1 min. Oxidized TMB (TMBox) underwent a color change from transparent to blue and then yellow by a stop solution. Moreover, the TMBox could be reduced on an indium tin oxide electrode, generating an electrochemical current, indicating that TMB can be used as a colorimetric and electrochemical indicator. The PBNPs modified with 3-aminophenylboronic acid (APBA) captured glycated albumin (GA) with a boronate affinity sandwich assay. As boronic acid binds to glycoproteins using cis-diol bonding, it can be used to detect GA. The APBA-modified PBNPs (PBBA) were involved with a sandwich complex formation and employed as nanozymes for the quantitative analysis of GA using colorimetric and electrochemical methods. Both methods showed strong linearities for different concentrations of GA. The results show that PBBA is a suitable alternative for natural enzymes and can be applied to sensitive determination of GA. [Display omitted] •Prussian blue nanozymes showed an excellent peroxidase-like activity.•Boronate affinity sandwich assays were developed to detect glycated albumin (GA).•The nanozymes were used in colorimetric and electrochemical assays of GA.•The developed assays were capable of accurate determination of GA in real samples.